4周间歇性禁食对大鼠骨骼肌质量及自噬的影响  

Effects of Four-week Intermittent Fasting on Skeletal Muscle Mass and Autophagy in Rats

作　　者：王祯[1] 于亮[1] 史霄雨 

机构地区：[1]北京体育大学运动人体科学学院,北京100084

出　　处：《中国运动医学杂志》2017年第8期680-686,共7页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金(31500964);霍英东教育基金会资助(151095);国家体育总局全民健身课题(2015B046);中央高校基本科研业务费专项资金资助;北京体育大学自主科研课题(2016RB018;2017YB028)

摘　　要：目的:探讨4周间歇性禁食后大鼠体重、体脂及骨骼肌质量的变化与大鼠骨骼肌自噬的关系,为间歇性禁食提供理论依据。方法:20只雄性SD大鼠随机分为对照组(Con组)和间歇性禁食组(IF组),每组各10只。采取每周三、周五间歇性禁食方案,每周按时记录大鼠体重,4周干预后双能X线吸收测量法(DEXA)分析体脂含量,之后分离双侧比目鱼肌称量湿重,免疫荧光法检测比目鱼肌laminin蛋白体现肌肉横截面积,透射电镜观察比目鱼肌自噬泡形态,Western Blot检测自噬相关蛋白LC3、p62及调控蛋白AMPKα、p-AMPKα、ULK1的表达情况。结果:4周间歇性禁食后,间歇性禁食组大鼠体重与体脂含量显著低于对照组(P<0.01),但各组比目鱼肌湿重及肌纤维横截面积并无明显差异(P>0.05)。间歇性禁食组比目鱼肌AMPKα、p-AMPKα、ULK1蛋白表达水平明显高于对照组(P<0.01),间歇性禁食组比目鱼肌内自噬标记LC3-Ⅱ/LC3-Ⅰ水平明显高于对照组,p62表达水平明显低于对照组,均具有非常显著性差异(P<0.01)。结论:4周间歇性禁食明显降低体脂水平,具有控制体重的作用,可激活AMPK-ULK1通路适度促进骨骼肌自噬,以维持骨骼肌质量,可作为一种潜在的"减肥"方法进行深入研究。Objective To observe the changes of body weight, fat mass and skeletal muscle mass of rats after 4 weeks of intermittent fasting, and explore relationship with autophagy in skeletal muscle, so as to provide theoretical basis for intermittent fasting. Method Twenty male Sprague Dawley rats were randomly divided into a control group （Con） and an intermittent fasting group （IF）,each of 10. The rats of IF group were forbidden to eat food every Wednesday and Friday, and the body weight of both groups was recorded weekly. After 4 weeks,Dual-Energy X-Ray Absorption （DEXA） was used to ana- lyze the body fat mass, then the bilateral soleus was separated to record the wet weight and measure the cross-sectional area of the soleus fibers by testing laminin with immunofluorescence confocal laser scanning microscope. The form of autophagic vacuole of soleus was observed using a transmission elec- tron microscopy. The expression of autophagy-related protein LC3,p62 and regulating protein AMPK,p- AMPK and ULK1 were measured using Western blotting. Result After 4 weeks of intermittent fasting, the weight and fat mass of IF were significantly lower than those of Con （P〈0.01）,but there were no significant differences between them in wet weight and cross-sectional area of soleus （P〉0.05）. The ex- pressions of AMPK,p-AMPK,ULK1 in IF were significantly higher than those in Con （P〈0.01）. Com- pared with Con, the expression of LC3-11/LC3- I of IF increased significantly, while the expression of p62 decreased significantly （P〈0.01）. Conclusion mass significantly, and control the weight efficiently Four weeks of intermittent fasting decreases the fat Intermittent fasting can maintain the skeletal mus- ele mass by promoting moderate autophagy through the AMPK-ULK1 pathway. It should be a potential ＂lose weight＂ method for further research.

关 键 词：间歇性禁食 骨骼肌质量 自噬 AMPK LC3 

分 类 号：R87[医药卫生—运动医学]