HPLC法测定不同产地灵芝中腺苷的含量  

Determination of Adenosine in Ganoderma Lucidum from Different Origins by HPLC

作　　者：来李娟 邓祖磊 葛德洲 刘浩[1] 赵丹[1] 

机构地区：[1]安徽省蚌埠医学院药学院,蚌埠233000 [2]安徽省蚌埠市食品药品检验中心,蚌埠233000

出　　处：《海峡药学》2017年第8期54-56,共3页Strait Pharmaceutical Journal

摘　　要：目的建立灵芝药材中腺苷的高效液相色谱含量测定方法,同时比较不同产地灵芝中腺苷含量的差异。方法采用HPLC法,色谱柱为AcclaimTM120-C18柱(4.6×150mm,5μm)、流动相为甲醇-0.01mol·L^(-1)磷酸二氢钾溶液(13∶87)、流速0.8m L·min^(-1)、检测波长260nm、柱温26℃、进样量5μL。结果灵芝中腺苷浓度在0.985~98.5μg·m L^(-1)(r=0.9999)范围内与峰面积积分值具有良好的线性关系;平均加样回收率为99.05%(RSD1.16%);方法精密度和重复性良好,RSD均小于2%。不同产地灵芝中腺苷的含量有较大差异。结论本法操作简单、结果准确、重现性好,为灵芝的质量控制提供参考方法。OBJECTIVE To establish an HPLC method for determination of adenosine in Ganoderma Lucidum, and simultaneously,to compare the contents of Ganoderma lucidum collected from different areas. METHODS The separation was peribrmed on an AcclaimTM 120-C18 column,with methanol-0. 01mol · L -1 potassium dihydrogen phos- phate solution（13：87） as the mobile phase at a flow rate of O. 8mL · min-l. The Detection wavelength was 260nm; the column temperature was 26℃ and the injection volume was 5μL. RESULTS It had a good linear relationship within the range of 0. 985 - 98.51mol · L -1（ r = 0. 9999 ） , and the average recovery rate is 99.05% （ RSD = 1.16% ）. And there were an obvious difference in the contents of Ganoderma lucidumfrom different are- as. CONCLUSION The method is simple, accurate and credible,that can provide a reference method for the quali- ty control of Ganoderma Lucidum.

关 键 词：灵芝 腺苷 高效液相色谱 含量测定 

分 类 号：R927.2[医药卫生—药学]