波长切换法测定车前子生品及盐炙品中3个成分的含量  

Contents Determination for Three Active Constituents of Plantaginis Semen Before and After Salt Processing by HPLC Wavelength Switching Method

作　　者：关书博[1] 王宇[2] 张振秋[2] 李可强 

机构地区：[1]沈阳市第二中医医院,辽宁沈阳110101 [2]辽宁中医药大学,辽宁沈阳110032 [3]辽宁省中药研究所,辽宁沈阳100161

出　　处：《辽宁中医杂志》2017年第8期1711-1715,共5页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:建立车前子生品及盐炙品中京尼平苷酸、毛蕊花糖苷和异毛蕊花糖苷3个成分的含量测定方法。方法:采用高效液相色谱法,色谱柱Agilent-C_(18)(4.6 mm×150 mm,5μm),以甲醇(A)-0.5%醋酸水(B)为流动相,梯度洗脱,流速1.0 m L·min^(-1)。结果:京尼平苷酸、毛蕊花糖苷和异毛蕊花糖苷浓度分别在0.007~350μg/m L;0.04~200μg/m L;0.09~450μg/m L范围内与峰面积呈现良好的线性关系。结论:该方法分离效率高,重复性好,可用于车前子药材的质量控制。Objective： To establish an HPLC method to determine the constituents,namely,geniposidic acid,verbascoside and isoverbascoside of Plantaginis Semen before and after salt processing. Methods： The Agilent-C_（18）（ 4. 6 mm × 150 mm,5 μm） was used with the mobile phase of methonal（ A）-0. 5% acetic acid aqueous（ B） for gradient elution,at the flow rate of 1. 0 m L/min. Results： The linear ranges of geniposidic acid,verbascoside and isoverbascoside were 0. 007-350 μg/m L,0. 04-200 μg/m L and 0. 09-450 μg/m L,respectively. The linear ranges of geniposidic acid,verbascoside and isoverbascoside were 0. 007-350 μg/m L,0. 04-200 μg/m L and 0. 09-450 μg/m L,respectively. Conclusion： This method,with high separation efficiency and good repeatability,can be used for the quality control of Plantaginis Semen.

关 键 词：车前子 京尼平苷酸 毛蕊花糖苷 异毛蕊花糖苷 

分 类 号：R282.710.3[医药卫生—中药学]