液质串联质谱法测定血液中异稻瘟净的不确定度评定  

Evaluation of Uncertainty for Determination of Iprobenfos in Human Blood by LC-MS/MS

作　　者：焦台风 欧军 程良红 

机构地区：[1]广东南天司法鉴定所法医毒物科室,广东深圳518000

出　　处：《广州化工》2017年第16期122-125,共4页GuangZhou Chemical Industry

摘　　要：液质串联质谱法测定血液中异稻瘟净的不确定度评定。对异稻瘟净浓度测定过程中各影响因素,包括测定精密度、称量、标准溶液的配制、含药血浆的配制、血浆提取、仪器、标准曲线拟合等进行分析评定,计算各变量的不确定度和合成不确定度,最终计算扩展不确定度。结论:液质串联质谱法测定血液中异稻瘟净的不确定度评定主要标准曲线拟合(尤其是低浓度)、标准溶液和血液样品制备处理及仪器允差引入。The uncertainty for the determination of Iprobenfos in human blood by LC-MS/MS was evaluated.Various factors affecting detection process,including repeatability,weighing,solution preparation,drug-spiked blood preparation,solid blood extraction,LC-MS/MS error,calibration curve fitting,and so on,were analyzed.The expanded uncertainty was obtained by calculating each component of uncertainty and combined uncertainty.When determining Iprobenfos in human blood by LC-MS/MS,the uncertainty was mainly caused by the standard curve fitting（ especially the low level of Iprobenfos）,solution and blood preparation,sample pretreatment and LC-MS/MS error.

关 键 词：药物分析 异稻瘟净 不确定度 

分 类 号：R917[医药卫生—药物分析学]