负压创面治疗术对大鼠脂肪干细胞迁移行为的影响  

Effect of negative-pressure wound therapy on adipose-derived stem cells migration behavior

作　　者：李晓明[1] 刘苹[1] 汪超[2] 杨佳佳[1] 姚仕采 张波[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所,重庆400042 [2]重庆医科大学附属儿童医院PICU/儿童发育疾病研究教育部重点实验室,重庆400014

出　　处：《重庆医学》2017年第25期3463-3465,3469,共4页Chongqing medicine

基　　金：中国科学院-威高集团高技术研究发展计划(ZKYWG2013-05)

摘　　要：目的探讨负压创面治疗术(NPWT)对大鼠脂肪干细胞(ADSCs)迁移行为的影响。方法 8头体质量为(20±2)kg巴马小香猪,分别接受假手术(A组,n=16)、持续负压吸引(B组,n=8)和间歇负压吸引(C组,n=8),治疗72h后观察3个月。细胞水平观察NPWT对ADSCs迁移行为的影响,提取大鼠ADSCs,采用8h持续负压吸引和间歇负压作用于ADSCs,Transwell检测ADSCs迁移能力,MTT检测细胞增殖能力,Western blot法检测缺氧诱导因子1α(HIF-1α)的表达。结果 B组和C组创面愈合效果明显优于A组;与A组比较,B、C组ADSCs细胞迁移数量差异均有统计学意义(P<0.05,P<0.01),而细胞增殖差异均无统计学意义(P>0.05);HIF-1α表达与ADSCs细胞迁移结果一致。结论 NPWT通过上调HIF-1α蛋白表达进而促进大鼠ADSCs迁移。Objective To investigate the effect of negative-pressure wound therapy （NPWT） on the migration behavior of rat adipose-derived stem cells （ADSCs）. Methods Eight （20 ± 2）kg Bama miniature pigs respectively received the sham operation （group A, n = 16）, continuous negative pressure suction （group B, n = 8） and intermittent negative pressure suction （group C, n = 8）. The pigs were observed for 3 months after 72 h treatment. The effect of NPWT on the migration behavior of ADSCs was ob- served at the cellular level. In order to avoid the influence of serum-free on cell viability,ADSCs were extracted and treated with continuous negative pressure suction or intermittent negative pressure suction for 8 h. The migration ability of ADSCs was detected by transwell. The proliferation ability of ADSCs was detected by MTT. The expression of hypoxia inducible factor-1α （HIF-1α） was detected by Western blot. Results The wound healing effect of continuous negative pressure suction group and intermittent nega- tive pressure suction group was better than that of the control group,and compared with the control group,the number of migrating ADSCs cells had statistically significant difference （P〈0.05,P〈0.01）, but cell proliferation had no statistically significant ＇difference. The expression of HIF-1α was consistent with the migration results of ADSCs. Conclusion NPWT can promote the migration of rat ADSCs by up-regulating HIF-1α protein expression.

关 键 词：负压创面治疗术 脂肪干细胞 迁移 

分 类 号：R641[医药卫生—外科学]