Rh血型抗原检测在精准化输血中的应用探讨  

Application of Rh antigens detection in precision blood transfusion

作　　者：程聪[1] 刘术臻[1] 邵长峰[1] 葛东梅[1] 闫松霞 王莉存[1] 孙波[1] 

机构地区：[1]青岛大学附属医院输血科,山东青岛266003

出　　处：《临床血液学杂志（输血与检验）》2017年第4期594-596,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:开展Rh血型抗原检测技术,利用实验室血型自动分析软件系统和输血信息管理软件系统等计算机信息识别技术,探讨Rh血型5种抗原检测在精准化输血中应用的可行性。方法:利用实验室血型全自动分析系统,对献血员红细胞标本和受血者标本都进行ABO血型和Rh 5种血型抗原(D、C、c、E和e)检测,将检测结果上传至输血信息管理系统,利用计算机识别技术为受血者自动匹配ABO血型和Rh 5种血型抗原相合的血袋,用凝聚胺法及抗人球蛋白卡式法进行交叉配血试验,实现临床精准化输血。结果:实施输血精准化方案后,90%以上的受血者可以实现ABO血型和Rh 5种血型抗原完全相合的输血,未见输血不良反应发生;与实施精准化输血方案前相比,多次输血或有输血史的患者血型不规则抗体阳性明显降低,且在不规则抗体阳性情况下疑难配血成功率大大提高。结论:开展Rh血型抗原检测技术实现临床精准化输血,既可以避免Rh系统抗体的产生,同时也减少了输血不良反应的发生;提升了解决疑难配血的能力,提高了输血治疗的安全性。Objective：To investigate the feasibility of precise blood transfusion compatible with ABO and five Rh antigens by the application of Rh antigen detection,the automatic analysis software for blood group and transfusion information management system.Method：Red blood cells（RBCs）from patients and donors were detected for ABO and Rh antigens（D,C,c,E and e）.The results were uploaded to transfusion information management system（TIMS）.The computer recognition technology automatically chose the appropriate blood compatible with ABO and Rh antigens.The blood cross-matching was done with Polybrene and Microcylinder Gel Card Antiglobulin method（MGC-AGH）to realize the precise blood transfusion.Result：Over 90 percent of patients could receive the blood completely compatible with ABO and five types of Rh antigens after the operation of the precision blood transfusion programme.All the blood transfusion was successful and no adverse transfusion reaction happened.The occurrence of irregular antibody in patients with repeated transfusion or transfusion history decreased significantly.The blood matching rate in patients with irregular antibody greatly increased.Conclusion：The detection of Rh antigens can promote the precise blood transfusion.It can help to avoid the occurrence of irregular antibody in Rh system and reduce the adverse transfusion reaction.The ability of problematic matching blood and safety of transfusion therapy were improved efficiently.

关 键 词：RH血型抗原 血型自动分析 精准化输血 输血治疗 安全性 

分 类 号：R457.1[医药卫生—治疗学]