黄连素对金黄色葡萄球菌急性肺损伤的保护作用及相关机制研究  

Protective Effect of Berberine on Staphylococcus Aureus induced A- cute Lung Injury and the Associated Immunological Mechanisms in Mice Model

作　　者：洪祝平 阙敢波[2] 丁先锋[1] 

机构地区：[1]浙江理工大学生命科学学院生物工程研究所,杭州市310018 [2]福建省龙岩市第二医院,福建省龙岩市364000

出　　处：《医学分子生物学杂志》2017年第3期152-156,共5页Journal of Medical Molecular Biology

基　　金：浙江省科技计划项目（No.2016C33228）

摘　　要：目的探讨黄连素（berberine，BBR）对金黄色葡萄球菌（Staphylococcus aureus，SA）诱导小鼠急性肺损伤的保护作用及相关机制。方法对小鼠行气道滴注sA构建急性肺损伤模型和体外刺激巨噬细胞RAW264．7感染模型。通过苏木精伊红染色方法（hematoxylin-eosin staining，HE）检测各组小鼠肺组织病理变化，Western印迹检测细胞中核转录因子P65蛋白和磷酸化分子p-P65蛋白，以及各样本中肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）和白介素_6（interleukin-6，IL-6）的表达以评估黄连素对SA诱导的急性肺损伤的作用。结果黄连素预处理可减轻SA诱导的急性肺损伤，减缓肺组织病理改变以及降低肺组织和细胞中炎性因子IL-6和TNF-α的表达并抑制p-P65蛋白的活化，但对P65的表达无影响。结论黄连素可通过抑制P65炎症信号通路的发生进一步抑制炎性因子的释放从而对SA诱导的急性肺损伤起到保护作用。Objective To explore the role of berberine in the treatment ofStaphylococcus aureus （SA） -induced acute lung injury in mice and the possible mechanisms. Methods The model of a- cute lung injury was established by intratraeheal instillation of SA, and the RAW264. 7 infection model was stimulated in vitro. The pathological changes of lung tissues, the expressions of Nuclear transcription factor （P65）, phosphorylated P65 （p-P65）, TNF-~ and IL-6 were measured to e- valuate the effect of berberine on acute lung injury. Results Pretreatment with berberine could sig- nificantly attenuate lung injury, improve the pathological changes of lung tissues, and reduce the expressions of p-P65, TNF-~ and IL-6 without influencing those of P65. Conclusion Berberin pro- tects against SA-induced acute lung injury by suppressing the P65 signal pathway mediated inflam- mation.

关 键 词：黄连素 金黄色葡萄球菌 急性肺损伤 

分 类 号：R563.1[医药卫生—呼吸系统]