HPLC法测定田参氨基酸胶囊中三七皂苷R_1、人参皂苷Rg_1、Re、Rb_1、Rd含量  

Determination of Notoginsenoside R_1,Ginsenoside Rg_1,Re,Rb_1 and Rd in Notoginsenoside-Ginseng Amino Acid Capsules by HPLC

作　　者：梁洪[1] 李波[1] 刘艳芳[1] 冯丽洁[1] 宋艳刚[1] 

机构地区：[1]丽珠集团利民制药厂,广东韶关512028

出　　处：《今日药学》2017年第8期528-531,共4页Pharmacy Today

摘　　要：目的建立测定田参氨基酸胶囊中药类成分含量的高效液相色谱(HPLC)法,以全面有效控制田参氨基酸胶囊质量。方法采用Phenomenex Kinetex C_(18)色谱柱(100 mm×4.6 mm,2.6μm);流动相:乙腈-水,梯度洗脱,流速:0.8 mL·min^(-1);检测波长:203 nm;柱温:30℃。结果三七皂苷R_1、人参皂苷Rg_1、Re、Rb_1、Rd线性范围分别为0.060 9~0.811 6,0.237 2~3.162 8,0.237 2~3.162 8,0.038 7~0.526 0,0.226 9~3.012 0,0.226 9~3.012 0和0.058 1~0.774 8 mg·mL^(-1)(r≥0.999 8);阴性无干扰;精密度、稳定性、重复性良好;回收率在93%~103%范围内。结论建立的方法准确、简便、重复性好,可用于田参氨基酸胶囊的质量控制。OBJECTIVE To establish a method for the determination of the content of the traditional Chinese medicine components in the Notoginsenoside-Ginseng amino acid capsules. METHODS The column was Phenomenex Kinetex C18column（ 100 mm×4.6 mm,2.6 μm）; with mobile phase of acetonitrile/water（ gradient elution） at a flow rate of 0.8 mL·min^（-1）,the detection wavelength was 203 nm,column temperature was 30 ℃. RESULTS The linear range was 0. 060 9-0. 811 6 mg·mL^（-1） for notoginsenoside R1,0.237 2-3.162 8 mg·mL^（-1） for ginsenoside Rg_1,0.038 7-0.526 0 mg·mL^（-1） for ginsenoside Re,0.226 9-3.012 0 mg·mL^（-1） for ginsenoside Rb1,0.058 1-0.774 8 mg·mL^（-1） for ginsenoside Rd. RSDs of precision,stability and reproducibility tests were lower than 3%,recoveries were 93%-103%. CONCLUSION The established HPLC method is accurate,simple and reproducible,and can be used for the quality control of Notoginsenoside-Ginseng amino acid capsules.

关 键 词：田参氨基酸胶囊 三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rd 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]