苦参碱对膀胱癌大鼠COX-2、cPLA2及PGDH蛋白表达水平的影响  

The Effect of Matrine on COX-2,cPLA2 and PGDH Protein Level in Rat with Bladder Cancer

作　　者：王明复 李艳秀 杨述海 

机构地区：[1]玉田县计划生育中心医院,河北玉田064100

出　　处：《中医学报》2017年第8期1363-1367,共5页Acta Chinese Medicine

基　　金：河北省科技计划项目(13277714D)

摘　　要：目的:探讨苦参碱对膀胱癌大鼠环加氧酶2(cyclooxygenase-2,COX-2)、细胞膜磷脂酶A2(cytosolic phospholipases A2,c PLA2)、前列腺素脱氢酶(prostaglandin dehydrogenase,PGDH)蛋白表达水平的影响。方法:选取SD雄性大鼠120只,随机分为正常对照组、模型组、塞来昔布组、苦参碱高剂量组、苦参碱中剂量组、苦参碱低剂量组,各20只。给予亚硝基胺灌胃建立膀胱癌模型,并给予相应的药物治疗。观察所有大鼠的膀胱质量、质量>200 mg膀胱所占比例、肿瘤细胞凋亡指数,并对各组大鼠COX-2、c PLA2及PGDH蛋白的表达水平进行检测。结果:治疗后,与模型组比较,各药物组大鼠的膀胱质量、质量>200 mg膀胱所占比例较低,其中塞来昔布组、苦参碱高剂量组低于苦参碱中剂量组、低剂量组(P<0.05);治疗后,与模型组比较,各药物组大鼠肿瘤细胞凋亡指数较高,其中塞来昔布组、苦参碱高剂量组高于苦参碱中剂量组、苦参碱低剂量组(P<0.05);治疗后,与模型组比较,各药物组大鼠COX-2阳性表达率较低,其中塞来昔布组、苦参碱高剂量组低于苦参碱中剂量组、苦参碱低剂量组(P<0.05);治疗后,与模型组比较,各药物组大鼠COX-2、c PLA2蛋白表达水平较低,其中塞来昔布组、苦参碱高剂量组低于苦参碱中剂量组、苦参碱低剂量组(P<0.05);各药物组大鼠PGDH蛋白表达水平较高,其中塞来昔布组、苦参碱高剂量组高于苦参碱中剂量组、苦参碱低剂量组(P<0.05)。结论:苦参碱能够明显抑制亚硝基胺诱发的大鼠膀胱癌的生长,其作用机制可能与抑制前列腺素通路相关蛋白COX-2、c PLA2表达、促进PGDH蛋白表达有关,且其作用具有剂量依赖性。Objective：To discuss the effect of Matrine on COX-2, cPLA2 and PGDH protein level in rat with bladder cancer. Meth- ods：120 SD male rats were randomly divided into normal control group, model group, celecoxib group, matrine high dose group, middle dose group and low dose group, each of 20 rats. Bladder cancer models were established by nitrosamine gastric perfusion in each group, and were given corresponding drug treatment. The bladder weight, proportion of bladder weight 〉 200 mg, tumor cell apoptosis index,and the expression levels of COX-2, cPLA2 and PGDH were detected. Results：After treatment, compared with model control group,the bladder weight and more than proportion 200mg of bladder weight of the rats in each group were lower, celecoxib group and matrine high dose group lower than matrine middle dose group and low dose group （ P 〈 0.05 ） ;the tumor cell apoptosis index of the tats in each group were higher, celecoxib group and matrine high dose group higher than matrine middle dosegroup and low dose group （P 〈 0.05 ） ; the positive expression rate of COX-2 of the rats in each group were lower, celecoxib group and matrine high dose group lower than matrine middle dose group and low dose group （P 〈 0.05 ） ; the expression levels of COX- 2 and cPLA2 protein of the rats in each group were lower, celecoxib group and matrine high dose group higher than matrine middle dose group and low dose group （P 〈 0.05） ; the expression levels of PGDH protein of the rats in each group were higher, celecoxib group and matrine high dose group higher than matrine middle dose group and low dose group （P 〈 0.05 ）. Conclusion ： Matrine can effectively inhibit the growth of bladder cancer induced by nitrosamine, and its mechanism is probably related with the expres- sion of prostaglandin pathway related proteins COX-2 and cPLA2, promoting the expression of PGDH protein, and the effect is dose dependent.

关 键 词：膀胱癌 苦参碱 亚硝基胺 COX-2蛋白 cPLA2蛋白 PGDH蛋白 大鼠 

分 类 号：R273.714[医药卫生—中西医结合]