特润舒都乐水母液浸膏对LPS诱导RAW264.7细胞TLR4/NF-κB信号通路及TNF-α表达的影响  

The Effect of TRSDL extract on the TLR4-NF-κB pathway and the TNF-α expression in RAW264.7 cells induced by LPS

作　　者：侯林[1] 薛海南 于峰[1] 王纯洁[2] 黄丽华[1] 

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014060 [2]内蒙古农业大学

出　　处：《包头医学院学报》2017年第8期75-77,101,共4页Journal of Baotou Medical College

基　　金：国家自然科学基金(NO31260590);内蒙古科技大学包头医学院博士启动基金(BSJJ201621)

摘　　要：目的:研究特润舒都乐水母液浸膏对LPS诱导的RAW264.7细胞的Toll样受体4(Toll like receptor 4,TLR4)、核转录因子-kappa B(nuclear factor-κB,NF-κB)以及肿瘤坏死因子(tumor necrosis factor-α,TNF-α)表达的影响。方法:采用脂多糖(lipopolysaccharide,LPS)刺激RAW264.7细胞,建立炎性细胞模型,采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)和逆转录-聚合酶链式反应法(reverse transcription-polymerase chain reaction,RT-PCR)分别从蛋白水平和mRNA水平检测TNF-α的表达情况;采用RT-PCR和westernblot方法检测TLR4和NF-κB的表达水平。结果:与空白对照组比较,LPS组RAW264.7细胞的TLR4、NF-κB和TNF-αmRNA表达均增加(P<0.05),与LPS组比较,各药物组TNF-α、TLR4及KF-κB mRNA表达水平均有所降低(P<0.05);与空白对照组比较,LPS组TNF-α、TLR4及NF-κB蛋白表达均增多(P<0.05),与LPS组比较,药物组上述指标的蛋白表达量均下降(P<0.05)。结论:特润舒都乐水母液浸膏发挥抗炎免疫作用的机制可能与其抑制TLR4/NFκB信号转导通路的激活,进而调节TNF-α基因表达有关。Objective： To study the influence of TRSDL extract on the Toll Like Receptor 4（ TLR4）,the expression of nuclear factor-κB（ NF-κB） and tumor necrosis factor-α（ TNF-α） in RAW264. 7 cells induced by LPS. Methods： Lipopolysaccharide（ LPS） were used to stimulate RAW264. 7 cells to make inflammatory cell models. Enzyme linked immunosorbent assay（ ELISA） and reverse transcription-polymerase chain reaction（ RT-PCR） were used to detect the expression of TNF-α from protein and mRNA levels; RT-PCR and westernblot were used to detect the expressions of TLR4 and NF-κB. Results： Compared with the control group,the expressions of TLR4,NF-κB and TNF-α mRNA in RAW264. 7 increased in the LPS group（ P〈0. 05）. Compared with the LPS group,the expressions of TNF-α,TLR4 and KF-κB mRNA decreased in each drug treated group（ P〈0. 05）. Compared with the control group,the expressions of TNF-α,TLR4 and NF-κB protein in the LPS group increased（ P〈0. 05）. Compared with the LPS group,the protein of the above indexes decreased in the drug treated group（ P〈0.05）. Conclusion ： The mechanism of antiinflammatory immune effect in TRSDLextract may be related to the inhibition of activating the TLR4/NFκB signal transduction pathway and the regulation of TNF-α gene expression.

关 键 词：特润舒都乐水母液浸膏 Toll样受体4 核转录因子-ΚB 肿瘤坏死因子-α 

分 类 号：R29[医药卫生—民族医学]