应用微纤维胶原支架构建组织工程软骨  

Tissue-engineered cartilage construction using microfibrillar collagen

作　　者：周丽斌[1] 徐冰心[2] 丁瑞英[2] 韩浩伦[2] 王刚[2] 李保卫[2] 王鸿南[2] 吴玮[2] 

机构地区：[1]广州医科大学附属口腔医院·广州口腔疾病研究所·口腔医学重点实验室,广东省广州市510140 [2]解放军第306医院耳鼻咽喉头颈外科,北京市100101

出　　处：《中国组织工程研究》2017年第22期3483-3487,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金青年科学基金项目(81401609)~~

摘　　要：背景:寻找理想的组织工程支架材料,仍然是软骨组织工程学的重要课题之一。目的:探讨微纤维胶原应用于软骨组织工程支架的可行性。方法:手术切取兔耳软骨,提取原代软骨细胞,并进行扩增培养。将海绵状微纤维胶原用无菌刀片切割成小方块,取第2代软骨细胞混悬液接种于微纤维胶原支架材料上。体外培养1周后,植入裸鼠体内,观察8周后取材,进行大体、组织学、免疫组织化学等方法观察。结果与结论:(1)在细胞接种过程中,湿水之后的微纤维胶原支架无明显缩水,仍然能够保持其立体形态;(2)体外培养1周以及植入体内8周后,无明显体积和形态变化;(3)裸鼠体内培养8周后形成白色半透明且富有弹性的成熟软骨块;(4)组织学观察可见所构建的软骨组织成熟,基质分泌旺盛,组织内仍可见未完全降解的微纤维胶原材料;(5)结果表明,微纤维胶原蛋白是一种良好的软骨组织工程支架材料。BACKGROUND： To seek for ideal scaffold materials is still an important task for cartilage tissue engineering. OBJECTIVE： To investigate the application of the Avitene？ microfibrillar collagen hemostat sponge in cartilage tissue engineering. METHODS： Rabbit auricular cartilage was harvested via surgical operation, and primary chondrocytes were isolated and amplified. Microfibrillar collagen hemostat sponge was cut into small bricks. The passage 2 chondrocytes were suspended and seeded onto the spongy bricks. After 1 week of in vitro culture, the constructs were then implanted into nude mice. After 8 weeks, the specimens were collected and evaluated using gross, histological and immunohistochamical observation. RESULTS AND CONCLUSION： During the cell seeding, the scaffold maintained its dimensions. No shrinkage was observed when the cell suspension was added. There was no considerable change in dimensions during the 1-week in vitro culture and at 8 weeks after implantation in nude mice. At 8 weeks post-implantation, mature cartilage blocks were harvested, which were white, translucent, and flexible. Histologically, the constructs appeared to have typical mature cartilaginous tissues, with robust extracellular matrix secretion, in which the microfibrillar collagen was incompletely degraded. We conclude that the microfibrillar collagen is a favorable scaffold material for cartilage tissue engineering.

关 键 词：生物材料 软骨生物材料 微纤维胶原 软骨细胞 软骨 组织工程 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]