钛芯与骨形成蛋白复合材料修复即刻种植牙槽骨缺陷的评价  

Titanium core/bone morphogenetic protein composite materials used to repair alveolar bone defects after immediate implant placement

作　　者：杨玉鹏[1] 赵海静[2] 谷建琦[1] 程凤峡[1] 郑瑶[1] 李娟[1] 郝玮[1] 王西西[1] 吴永生[1] 

机构地区：[1]河北省人民医院口腔科,河北省石家庄市050051 [2]河北省人民医院老干部处,河北省石家庄市050051

出　　处：《中国组织工程研究》2017年第22期3536-3540,共5页Chinese Journal of Tissue Engineering Research

基　　金：2014年度河北省医学科学研究课题计划(ZL20140216)~~

摘　　要：背景:致力于种植牙槽骨缺损修复的实验和临床研究是现代科技发展方向,寻找适合的骨替代材料成为人们研究的热门课题。目的:研究钛芯羟基磷灰石与骨形成蛋白复合材料修复即刻种植后牙槽骨缺陷的效果。方法:将24只新西兰兔随机分为3组,正常组不进行任何处理,实验组、对照组制作股骨大转子区骨缺损模型,实验组于骨缺损处植入种植体及钛芯羟基磷灰石与骨形成蛋白复合材料;对照组植入种植体及钛芯羟基磷灰石材料。植入后4周,检测血CD4^+、CD8^+淋巴细胞比例及NK细胞活性、白细胞介素2水平;植入后16周,采用X射线检测骨密度,组织学观察种植体周围成骨情况。结果与结论:(1)实验组骨密度高于对照组(P<0.05);(2)实验组复合材料周围有不同程度的裂解,新生骨组织细胞和基质细胞较为丰富,并且在种植体周围有良好的骨结合接触,内部出现有红染成熟骨组织进入;对照组骨细胞数量、纤维细胞增生少于实验组;(3)实验组CD4^+与CD8^+淋巴细胞比例、NK细胞活性、白细胞介素2水平低于对照组(P<0.05);(4)结果表明,钛芯羟基磷灰石与骨形成蛋白复合材料能够有效修复即刻种植后牙槽骨缺陷区域,引导骨细胞生长。BACKGROUND： Now experimental and clinical research on suitable bone substitutes for alveolar bone defects after dental implantation is an issue of concern. OBJECTIVE： To study the therapeutic effect of titanium core/bone morphogenetic protein （BMP） composite material on alveolar bone defects after immediate implant placement. METHODS： Twenty-four New Zealand rabbits were randomly assigned into normal group （no intervention）, experimental group or control group. Animal models of bone femoral greater trochanter defect were made in the experimental and control groups. Dental implant and titanium core/BMP composite material were implanted in the experimental group, while dental implant and titanium core were implanted in the control group. Percentage of CD4＋, CD8＋ T lymphocytes, natural killer cell activity and interleukin 2 level were detected at postoperative 4 weeks; bone mineral density and osteogenesis around the implant were detected at postoperative 16 weeks through X-ray and histological examinations. RESULTS AND CONCLUSION： X-ray results showed that the bone mineral density in the experimental group was significantly higher than that in the control group （P 〈 0.05）. Histological results showed that in the experimental group, different degrees of cell lysis around the composite, more bone cells and bone matrices were found, implant-bone osseointegration formed well, and red-dyed mature bone tissues were detective inside the implant. Compared with the experimental group, lower number of bone cells and fibrocytes were found in the control group. Additionally, the percentage of CD4＋ and CD8＋ T lymphocytes, natural killer cell activity and interleukin 2 level in the experimental group were significantly lower than those in the control group （P 〈 0.05）. To conclude, the titanium core/BMP composite material can effectively repair alveolar bone defects after immediate implant placement to guide the growth of bone cells.

关 键 词：生物材料 口腔生物材料 钛芯 骨形成蛋白 复合材料 即刻种植 牙槽骨 兔 缺陷 骨密度 生物相容性 炎症反应 

分 类 号：R318[医药卫生—生物医学工程]