改良纳米羟基磷灰石/聚乙烯醇多孔复合水凝胶人工角膜材料的生物相容性  

Biocompatibility of modified nano-hydroxyapatite/polyvinyl alcohol porous composite hydrogel as an artificial corneal material

作　　者：杜倩[1] 杜琛 金贵玉[1] 田华[1] 

机构地区：[1]南阳市中心医院眼科,河南省南阳市473000 [2]唐河县城郊医院公共卫生服务科,河南省南阳市473000

出　　处：《中国组织工程研究》2017年第22期3541-3546,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:如何提高人工角膜在宿主体内的稳定性,减少并发症仍是当前需要解决的关键问题。因此寻找一种理想的生物相容性高的支架材料仍是研究的重点。目的:探讨改良纳米羟基磷灰石/聚乙烯醇多孔复合物水凝胶和单纯聚乙烯醇水凝胶作为人工角膜材料的生物相容性。方法:(1)倒置显微镜观察改良纳米羟基磷灰石/聚乙烯醇和单纯聚乙烯醇提取液培养48 h后兔角膜基质成纤维细胞生长情况;(2)MTT法检测改良纳米羟基磷灰石/聚乙烯醇和单纯聚乙烯醇提取液培养不同时间兔角膜基质成纤维细胞的相对增殖率;(3)新西兰白兔进行人工角膜移植实验,于植入48 h后观察过敏、热原反应以及死亡情况;(4)人工角膜植入4周后取角膜进行苏木精-伊红染色;(5)人工角膜植入4周后,采用ELISA检测血清炎症因子水平;(6)人工角膜植入1-4周,裂隙灯观察角膜、结膜充血水肿、角膜新生血管变化,并统计新生血管出现时间以及新生血管生长面积评分。结果与结论:(1)与空白对照组相比,加入2种人工角膜材料提取液培养细胞48 h后,细胞均稀疏,生长速度缓慢,改良纳米羟基磷灰石/聚乙烯醇组细胞较聚乙烯醇组生长较快,呈梭形,拉丝现象不明显;(2)与空白对照组相比,加入2种材料提取液培养细胞不同时间后,细胞相对增殖率均明显降低(P<0.05),改良纳米羟基磷灰石/聚乙烯醇组的细胞增殖率高于聚乙烯醇组(P<0.05);(3)植入2种材料48 h后,改良纳米羟基磷灰石/聚乙烯醇组仅有2只兔子发生过敏反应,无热原反应和死亡,而聚乙烯醇组有2只兔发生过敏反应,2只发生热原反应,无死亡;(4)植入4周后,与空白对照组比较,两组兔血清炎性因子水平明显增加(P<0.05),改良纳米羟基磷灰石/聚乙烯醇组的炎性因子水平少于聚乙烯醇组(P<0.05);(5)改良纳米羟基磷灰石/聚乙烯醇组新生血管出现时间晚于聚乙烯醇组,新生血管BACKGROUND： How to improve the stability of artificial cornea in the host and reduce the complications is the current key issues to be solved. Therefore, looking for an ideal biocompatible scaffold material is still the focus of the study. OBJECTIVE： To explore the biocompatibility of nano-hydroxyapatite （nHA）/polyvinyl alcohol （PVA） porous composite hydrogel and pure PVA hydrogel as artificial corneal materials. METHODS： Inverted microscope was used to observe cell growth of rabbit corneal stromal fibroblasts when cultured with nHA/PVA composite hydrogel extract or PVA extract for 48 hours. MTT method was used to detect the relative proliferation rate of rabbit corneal stromal fibroblasts cultured with nHA/PVA composite hydrogel extract or PVA extract. Systemic toxicity, allergies, pyrogen reaction and deaths were observed in New Zealand white rabbits at 48 hours after artificial corneal implantation. ELISA and hematoxylin-eosin staining were used to detect changes in serum inflammatory factors at 4 weeks after artificial corneal implantation. Slit-lamp examination was performed to observe corneal or conjunctival hyperemia/edema and corneal neovascularization at 1-4 weeks after corneal implantation. Corneal neovascularization time and neovascularization area were detected after the two materials were implanted. RESULTS AND CONCLUSION： The cells were sparse and grew slowly at 48 hours after culture in nHA/PVA composite hydrogel or PVA extract as compared with the blank control group. The cell growth in the nHA/PVA composite hydrogel group was better than that in the PVA group. The relative cell proliferation rate was significantly decreased at different time after culture in nHA/PVA composite hydrogel or PVA extract compared with the blank control group （P 〈 0.05）, and the relative cell proliferation rate in the nHA/PVA composite hydrogel group was significantly higher than that in the PVA group （P 〈 0.05）. There were two rabbits appearing to have allergic reaction, but no one prese

关 键 词：生物材料 材料相容性 纳米羟基磷灰石 聚乙烯醇 水凝胶 人工角膜 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]