流体剪切力作用下阿司匹林对不同钛表面MG-63细胞增殖活性的影响  

Aspirin effects on MG-63 cell proliferation on different modified titanium surfaces under fluid shear stress

作　　者：梁仲朗 刘长虹 李世轶[1] 杨晓喻[1] 

机构地区：[1]南方医科大学口腔医院.广东省口腔医院种植中心,广东省广州市510260 [2]广东协大口腔医院,广东省广州市510399

出　　处：《中国组织工程研究》2017年第22期3555-3560,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(30500569)~~

摘　　要：背景:最近有国内外临床试验、动物实验和体外细胞学实验证明,适宜浓度阿司匹林能上调成骨细胞的增殖及成骨功能。目的:探讨流体剪切力作用下不同浓度阿司匹林对成骨细胞增殖的影响。方法:(1)采用含体积分数10%胎牛血清与不同浓度阿司匹林(0,0.023,0.046,0.062 5,0.125,0.25,0.5,1.0,2.0,4.0 mmol/L)的DMEM低糖培养液培养MG-63人成骨样细胞,1-7 d后,MTS法检测细胞增殖;(2)将MG-63人成骨样细胞分别接种于高度抛光钛板、喷砂酸碱处理钛板及光滑载玻片上,培养1 d后,再分别以含不同浓度阿司匹林(0,0.5 mmol/L)的培养液培养3 d,施加流体剪切力,加力0,0.5,1,2,4 h后,MTS法检测细胞增殖。结果与结论:(1)不同浓度阿司匹林对细胞增殖的作用:培养1-3 d时,0.023,0.046,0.062 5,0.5 mmol/L的阿司匹林可促进MG-63人成骨样细胞的增殖;培养1-7 d,1,2,4 mmol/L的阿司匹林抑制MG-63人成骨样细胞的增殖;(2)流体剪切力作用下阿司匹林对细胞增殖的作用:单因素方差分析结果显示,阿司匹林药物处理因素对细胞增殖影响的差异有统计学意义(F=8.349,P=0.004),0.5 mmol/L阿司匹林可降低MG-63人成骨样细胞的增殖活性;材料表面处理对细胞增殖影响的差异无统计学意义(F=2.826,P=0.064);不同流体剪切力加载时间对细胞增殖影响的差异无统计学意义(F=0.893,P=0.406);(3)流体剪切力下0.5 mmol/L阿司匹林对细胞增殖的作用:材料表面处理对细胞增殖影响的差异无统计学意义(F=1.803,P=0.171);0.5 mmol/L阿司匹林可显著抑制玻片组MG-63人成骨样细胞的增殖(P=0.003),对高度抛光钛板及喷砂酸碱处理钛板组MG-63人成骨样细胞增殖无显著抑制作用(P=0.891,P=0.051);(4)结果表明:不同浓度阿司匹林对MG-63人成骨样细胞增殖有明确的影响作用,且有浓度依赖性;不同钛表面处理可降低阿司匹林对MG-63人成骨样细胞增殖的影响作用。BACKGROUND： Recent research has shown that the proper concentration of aspirin can increase the proliferation and osteogenic ability of MG-63 cells. OBJECTIVE： To explore the effects of different concentrations of aspirin on osteoblast proliferation on the implant-cell interface under fluid shear stress. METHODS： （1） MG-63 cells were cultured in low-glucose DMEM containing 10% fetal bovine serum and different concentrations of aspirin （0, 0.023, 0.046, 0.0625, 0.125, 0.25, 0.5, 1.0, 2.0, 4.0 mmol/L） for 1-7 days. Then cell proliferation was detected using MTS method. （2） MG-63 cells were cultured on three different surfaces： glass slide, PT titanium surface and SLA titanium surface. After 3 days of culture with aspirin at a concentration of 0 or 0.5 mmol/L, the cells were subjected to fluid shear stress. MTS test was applied to estimate the proliferation of MG-63 cells at 0, 0.5, 1, 2, 4 hours after stress application. RESULTS AND CONCLUSION： （1） After 1-3 days of culture, 0.023, 0.046, 0.5 mmol/L aspirin promoted the proliferation of MG-63 cells, while after 1-7 days of culture, 1, 2, 4 mmol/L aspirin inhibited the proliferation of MG-63 cells. （2） Under the fluid shear stress, aspirin showed significant effects on the cell proliferation as confirmed by one-way analysis of variance （F=8.349, P=0.004）, and 0.5 mmol/L aspirin inhibited the cellular proliferation of MG-63 cells. However, surface modification and stress loading time showed no significant effects on the cell proliferation （F=2.826, P=0.064; F=0.893, P=0.406）. （3） Under the fluid shear stress, surface modification showed no significant effect on the cell proliferation of MG-63 cells cultured with 0.5 mmol/L aspirin （F=1.803, P=0.171）. Under the fluid shear stress, 0.5 mmol/L aspirin significantly inhibited the proliferation of MG-63 cells on the glass slide （P=0.003）, while PT and SLA titanium surfaces showed on inhibitory effect on the cell proliferation （P=0.891, P=0.051）. The present results

关 键 词：生物材料 材料相容性 阿司匹林 流体剪切力 钛表面 表面形貌 成骨细胞 非类固醇类抗炎药 增殖活性 骨结合 旧药新用 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]