HPLC法同时测定维药驱虫斑鸠菊中4个黄酮类成分含量  

Simultaneous Determination of Four Flavonoids in Vernonia Anthelmintica Willd. by HPLC

作　　者：王加利[1] 袁将 全庆华 姬瑞芳 刘永刚[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《辽宁中医药大学学报》2017年第8期43-45,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30901959);新疆自然基金项目(201318101-5)

摘　　要：目的:建立高效液相色谱法同时测定维药驱虫斑鸠菊中圣草酚、紫铆查耳酮、紫铆素和异鼠李素含量的方法,为完善驱虫斑鸠菊质量标准提供依据。方法:以圣草酚、紫铆查耳酮、紫铆素和异鼠李素为对照品,采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈为流动相A,0.2%磷酸水溶液为流动相B,进行梯度洗脱,检测波长310 nm,流速1.0 m L·min-1,柱温25℃,测定驱虫斑鸠菊药材中这4个黄酮类成分的含量。结果:本法中圣草酚、紫铆查耳酮、紫铆素和异鼠李素的进样量分别在13.44~107.52、30.24~241.92、9.60~76.80、6.25~49.97 ng范围内与峰面积积分值呈良好线性关系,r分别为0.9998、0.9999、0.9991、0.9997,平均加样回收率(n=9)在99.70%~100.56%,RSD小于2.0%。结论:本法简单、快速、重现性好,可为评价驱虫斑鸠菊药材的质量提供依据。Objective： This study is to establish a method for simultaneous determination of eriodictyol, butein, hutin and isorhamnetin in Vernonia anthelmintica Willd. by HPLC, a kind of Uyghur medicine, which can provide the basis for quality standard of Vernonia anthelmintica Willd. Methods： Eriodictyol, butein, butin and isorhamnetin were reference substance. The analysis was performed on a ZORBAX SB-C18 column （4.6 mm × 250 mm, 5 μ m ）with the mobile phase consisted of mobile phase A （ acetonitrile ） and mobile phase B （ 0.2% phosphoric acid solution ） in a programme of gradient elution. The detection wavelength was 310 nm, the flow rate was 1.0 mL · min^-1 and the column temperature was 25 ℃. Results ： The linear ranges of eriodictyol, butein, butin and isorhamnetin were 13.44-107.52,30.24-241.92,9.60-76.80,6.25-49.97 ng. The average recovery rates （ n=9 ） for the sample preparation of the markers were in the range of 99.70%-100.56% and RSD was less than 2.0%. Conclusion： The method is simple, rapid and replicable, which can be used for the quality control of Vernonia anthelmintica Willd.

关 键 词：驱虫斑鸠菊 圣草酚 紫铆查耳酮 紫铆素 异鼠李素 黄酮类化合物 含量测定 

分 类 号：R758.410.5[医药卫生—皮肤病学与性病学]