松嫩平原盐碱土AM真菌对白花三叶草生长及生理生化的影响  

Effect of AMF on Growth and Physio-biochemistry of Trifolium repens under Stress of Salt Alkaline Soil in Songnen Plain

作　　者：黄寿臣 陈飞[1] 李丽丽[1] 杨春雪[1] 

机构地区：[1]东北林业大学园林学院,黑龙江哈尔滨150040

出　　处：《贵州农业科学》2017年第7期61-67,共7页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"星星草-丛枝菌根共生体应答盐碱逆境的生理机制解析"(31601986);中央高校基本科研业务费专项资金项目"松嫩盐碱草地星星草根围AM真菌多样性与功能研究"(2572015CA22);东北油田盐碱植被恢复与重建教育部重点实验室开放基金项目"松嫩盐碱草地马蔺根围AM真菌多样性研究"(SAVER1608);黑龙江省博士后科研启动基金"松嫩盐碱草地典型耐盐园林花卉根围AM真菌多样性研究"(LBH-Q16005)

摘　　要：为松嫩盐碱草地的改良提供理论依据,以白花三叶草为宿主植物,模拟松嫩盐碱草地土壤盐碱成分及其含量,设置盐含量为0.197%、0.394%、0.788%和1.182%的4个复合盐碱梯度,探究根内根生囊霉、幼套近明囊霉以及混合接种对复合盐碱胁迫下白花三叶草生长生理的影响。结果表明:AM真菌能与白花三叶草形成共生体系,增加其生长量;AM真菌通过增加植物组织含水量,降低水分饱和亏改善白花三叶草水分利用效率;接种AM真菌后SOD、POD、CAT 3种防御酶活性明显提高,也相应增加了可溶性蛋白及叶绿素含量,且接种根内根生囊霉对白花三叶草的促进效果最明显。In order to provide the theory basis for the improvement and restoration of degraded Songnen saline grassland, T.repens was selected as a host plant in this study.Four kinds of composite salt concentration which were 0.197%, 0.394%, 0.788%, 1.182% were set up, so as to simulate the salt and alkali content in Songnen alkaline grassland.In this paper the authors explored the effects of Rhizophagus intraradices, Claroideoglomus etunicatum and the mixed agents of the above two on the growth and physiological characteristics of T.repens under saline alkali stress.Results： AMF was able to form a good symbiotic system with T.repens and increase the biomass.It also improved the plant water status by enhancing tissue water content and abating water saturation deficit, increased antioxidant enzyme activity such as SOD, POD, CAT as well.In addition to these aspects, it also had positive effect in improving the soluble protein content and chlorophyll content.The positive effect of T.repens inoculated by R.intraradices was the best.

关 键 词：丛枝菌根(AM)真菌 白花三叶草 复合盐碱胁迫 

分 类 号：S46[农业科学—植物保护]