不同产地和叶形附子水煎液的抗炎、镇痛作用比较  

Comparison of the Anti-inflammation,Analgesic Effects of Decoctions Extracted from Aconiti Lateralis with Different Leaf Shapes from Different Producing Areas

作　　者：缪璐琳 熊秋韵 高继海[1] 李梦婷[1] 敖慧[1] 谢晓芳[1] 彭成[1] 

机构地区：[1]成都中医药大学药学院,成都611137

出　　处：《中国药房》2017年第25期3483-3486,共4页China Pharmacy

基　　金：国家基础科学人才培养基金资助项目(No.J1310034);国家自然科学基金资助项目(No.81630101);国家中药标准化项目(No.ZYBZH-C-SC-51);四川省中医药科学技术研究专项项目(No.2016ZY008);四川省科技厅项目(No.2013SZ0114);四川省青年科技创新研究团队专项计划项目(No.2015TD0028);四川省科技计划项目(No.2014SZ0156)

摘　　要：目的:比较不同产地(江油、布拖)和叶形(大花叶、小花叶)附子水煎液的抗炎、镇痛作用。方法:将动物随机分为空白组(蒸馏水),阳性组,江油大、小花叶附子水煎液组和布拖大、小花叶附子水煎液组(给药剂量按生药计均为5 g/kg)。分别通过二甲苯致小鼠耳廓肿胀实验(n=12)和鸡蛋清致大鼠足跖肿胀实验(n=10)(阳性药物均为醋酸地塞米松片,0.005 g/kg)考察不同产地和叶形附子水煎液的抗炎作用;分别通过冰醋酸致小鼠扭体反应实验(n=12)和小鼠热板致痛实验(n=12)(阳性药物均为盐酸吗啡片,0.002 5 g/kg)考察不同产地和叶形附子水煎液的镇痛作用。结果:布拖大、小花叶附子和江油小花叶附子水煎液均可显著降低小鼠的耳廓肿胀度(P<0.01);江油大花叶附子水煎液和布拖大花附子水煎液可显著降低给药6 h后大鼠的足跖肿胀度(P<0.05);布拖小花叶附子水煎液可显著减少醋酸致痛小鼠的扭体次数、延长热板致痛小鼠的痛阈值(P<0.05或P<0.01)。结论:布拖大花叶、小花叶附子和江油小花叶附子的抗炎作用较好,布拖小花叶附子的镇痛作用更佳。OBJECTIVE： To compare the anti-inflammation, analgesia effects of decoctions extracted from Aconiti lateralis with different leaf shapes （dahua leaf, xiaohua leaf） from different producing areas （Jiangyou, Butuo）. METHODS： Animals were randomly divided into blank group （distilled water）, positive group, groups of Aconiti lateralis with dahua, xiaohua leaf from Ji- angyou, groups of Aconiti lateralis with dahua, xiaohua leaf from Butuo （with dose of 5 g/kg, calculated by crude drug）. The an- ti-inflammation effect of decoctions extracted from Aconiti lateralis with different variety sources and leaf shapes was investigated by xylene-induced ear swelling test （n=12） in mice and egg white-induced toe swelling test （n=10） in rats （positive drug was Dexamethasone acetate tablet, 0.005 g/kg）. And its analgesic effect was investigated by acetic acid-induced writhing body reaction test （n= 12） and hot-plate-induced pain test （n----12） in mice （positive drug was Morphine hydrochloride tablet, 0.002 5 g/kg）. RE- SULTS： The decoctions extracted from Aconiti lateralis with dahua, xiaohua leaf from Butuo and xiaohua leaf from Jiangyou can significantly reduce the ear swelling degree （P〈0.01）. The decoctions extracted from Aconiti lateralis with dahua leaf from Jiangy- ou and Butuo can significantly decrease the toe swelling degree after 6 h of medieation （P〈0.05）. And decoctions extracted from Aconiti lateralis with xiaohua leaf from Butuo can significantly reduce the number of writhing body in mice with acetic acid-in- duced pain and prolong the pain threshold of mice with hot-plate-induced pain （P〈0.05 or P〈0.01）. CONCLUSIONS： Aconiti lateralis with dahua and xiaohua leaf from Butuo and with xiaohua leaf from Jiangyou show better anti-inflammation effect, and Aconiti lateralis with xiaohua leaf from Butuo shows better analgesic effect.

关 键 词：产地 附子 叶形 抗炎 镇痛 

分 类 号：R285[医药卫生—中药学] R282.5[医药卫生—中医学]