苗药了哥王不同炮制品乙醇提取物对小鼠的急性毒性作用比较  

Comparison of Acute Toxicity Effects of Ethanol Extract from Different Processed Products of Miao Medicine Wikstroemia indica on Mice

作　　者：冯果[1,2,3] 李玮[1,3] 何新[2] 郑传奇[1] 冷安碧 田小芳[1] 

机构地区：[1]贵阳中医学院药学院,贵阳550025 [2]天津中医药大学中药学院,天津300193 [3]国家苗药工程技术研究中心,贵阳550025

出　　处：《中国药房》2017年第25期3536-3540,共5页China Pharmacy

基　　金：国家中医药管理局中医药部门公共卫生服务项目(No.国中医药科技中药便函[2015]132号);国家级大学生创新创业训练计划项目(No.201610662025);贵阳中医学院基金项目(No.贵中医科院内[2016]40号);贵州省高校工程技术研究中心建设项目(No.黔教合KY字[2012]021号)

摘　　要：目的:比较苗药了哥王生品、不同炮制品乙醇提取物对小鼠的急性毒性,为了哥王汗渍法炮制工艺优选及其安全用药提供依据。方法:采用汗渍法对了哥王的饮片炮制30d得炮制品一,对了哥王粗粉分别炮制14、7d得炮制品二和炮制品三。以70%乙醇为溶剂,采用渗漉法对了哥王生品及不同炮制品进行提取,并对不同乙醇提取物进行小鼠急性毒性实验。结果:了哥王生品、炮制品一乙醇提取物的半数致死量(LD_(50))分别为4.05、6.65 g/kg,相当于临床70kg成人日用量的19、32倍。而了哥王炮制品二、炮制品三乙醇提取物的LD50无法测出;最大耐受量(MTD)分别为20.0、15.0 g/kg,相当于临床70 kg成人日用量的95、71倍;最大给药量(MLD)分别近似为30.0、20.0 g/kg,相当于临床70kg成人日用量的143、95倍。结论:了哥王炮制品的毒性明显低于生品,且了歌王粗粉炮制14d后毒性低于炮制7d。OBJECTIVE： To compare the acute toxicity of ethanol extract from raw product and different processed products of Wikstroemia indica on mice, and provide basis for optimizing the processing technology of perspiration method for W. indica and medication safety. METHODS： Perspiration method was used to process the /（. indica pieces for 30 d to get processed product 1 and process its coarse powder for 14, 7 d to get processed product 2, processed product 3, respectively. Then using 70% ethanol as solvent, percolation method was used to extract the raw W. indica and its different processed products, and acute toxicity test was conducted on mice for different ethanol extracts. RESULTS： The median lethal dose （LD50） of ethanol extracts from raw W. in- dica and processed product 1 were 4.05 and 6.65 g/kg, equivalent to 19, 32 times of the clinical daily dose of a 70 kg adult, re- spectively. While the LDs0 of ethanol extract from processed product 2 and processed product 3 can not be measured, the maximtLrn tolerated dose （MTD） were measured as 20.0, 15.0 g/kg, equivalent to 95, 71 times of the clinical daily dose of a 70 kg adult, re- spectively; the maximum dose （MLD） were measured as approximately 30.0, 20.0 g/kg, equivalent to 143, 95 times of the clini- cal daily dose of a 70 kg adult, respectively. CONCLUSIONS： The toxicity of processed products of W, indica is obviously lower than that of raw products, and its toxicity after processing the coarse powder for 14 d is lower than that after processing the coarse powder for 7 d.

关 键 词：了哥王 汗渍法 炮制 急性毒性 小鼠 

分 类 号：R285[医药卫生—中药学]