X射线辐照处理对甘草三萜类及黄酮类有效成分含量的影响  

Effect of X-ray Irradiation Treatment on Contents of Triterpenoids and Flavonoids in Glycyrrhizae Radix et Rhizoma

作　　者：胡婷[1] 高智强[1] 马永生[1] 周姗[1] 尹彦超[1] 张晓冬[1] 李文东[2] 刘颖[1] 

机构地区：[1]北京中医药大学生命科学学院,北京100102 [2]北京市药品检验所北京市保健食品化妆品检验中心,北京102206

出　　处：《中国实验方剂学杂志》2017年第17期17-21,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81503181)

摘　　要：目的:探讨X射线辐照处理对乌拉尔甘草中三萜类及黄酮类有效成分含量的影响,提高栽培甘草的突变率,为甘草的分子育种奠定基础。方法:分别采用不同辐射剂量的X射线对甘草种子进行辐照处理,栽培1年后采收。采用HPLC测定甘草样品中2种三萜类成分(甘草酸、异甘草酸)及4种黄酮类成分(甘草苷、异甘草苷、甘草素及异甘草素)的含量,计算各成分产量,应用非参数检验进行统计学分析。结果:X射线辐照样品中三萜类和黄酮类有效成分的含量及产量均高于空白组。各有效成分产量随辐射剂量增加而呈先上升后下降再上升趋势。当辐射剂量为5 Gy时,各化合物的产量与空白组相比显著提高;当辐射剂量增加至10 Gy时,各化合物产量有所下降;当辐射剂量增加至20 Gy时,化合物产量再次明显提高,并在辐射剂量为50 Gy时达到最大值。结论:X射线辐照处理能增加甘草生物量及其有效物质的含量和产量,其机制可能是X射线引起甘草基因突变,从而引起代谢物的变化。Objective： To investigate effect of X-ray irradiation on the contents of triterpenoids and flavonoids in Glycyrrhizae Radix et Rhizoma for increasing the mutation rate of cultivated product of this herb, and lay a foundation for molecular breeding of Glycyrrhizae Radix et Rhizoma. Method ： Different radiation doses of X- ray were used to irradiate the seeds of Glycyrrhizae Radix et Rhizoma, which were cultivated for one year. The contents of two triterpenoids and four flavonoids were assayed by HPLC. And nonparametric test were used to analyze the contents and yields of these six components. Result： The contents and yields of these six components in radiation samples were higher than that in blank group. The yields of triterpenoids and flavonoids showed an increasing-decreasing-increasing pattern with the increase of radiation dose. Compared with the blank group, the yields of six components in radiation group increased dramatically at the radiation dose of 5 Gy, and declined slightly at the radiation dose of 10 Gy, then increased again at the radiation dose of 20 Gy, and reached the peak at the radiation dose of 50 Gy. Conclusion： X-ray irradiation treatment can increase the biomass of Glycyrrhizae Radix et Rhizoma and improve the contents and yields of six active components, its mechanism may be associated with gene mutation, which changes the accumulation of metabolites.

关 键 词：甘草 X射线 甘草苷 异甘草苷 甘草素 异甘草素 甘草酸 异甘草酸 

分 类 号：R282[医药卫生—中药学] O348.6[医药卫生—中医学]