道地药材川麦冬和浙麦冬的生态遗传分化  

Ecogenetic and Differentiation of Genuine Medicinal Materials:Ophiopogonis Radix in Sichuan and Zhejiang Province

作　　者：刘霞[1,2] 林韵涵[2] 谢彩香[3] 曾凡琳[3] 马晓冲[3] 孙伟[1] 屠鹏飞[4] 陈士林[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]武汉理工大学化学化工与生命科学学院,武汉430070 [3]中国医学科学院药用植物研究所,北京100193 [4]北京大学药学院,北京100191

出　　处：《中国实验方剂学杂志》2017年第17期27-33,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2014ZX09304307001);国家自然科学基金重点项目(8113006);国家中药基因库建设项目(G2016001)

摘　　要：目的:探讨川麦冬和浙麦冬的生态型差异,为道地药材生物学实质的深入研究提供参考。方法:选择传统道地药材麦冬为模型,收集75份实验样本,通过分子鉴定技术和生态因子分析研究川麦冬和浙麦冬的生态型差异。结果:川麦冬和浙麦冬的psbA-trnH序列在49位点处存在变异,川麦冬为碱基腺嘌呤(A),浙麦冬为碱基鸟嘌呤(G),此为两者序列的差异化特征;生态因子分析显示四川、浙江两大产区呈现不同的地理生态型。其中,浙江产区在年均日照强度、年均降水量、年均相对湿度、最热季平均温度、生长期平均温度等因子上高于四川,而四川产区的最冷季平均温度高于浙江。结论:川麦冬和浙麦冬的基因型和生态因子均存在明显差异。Objective： To investigate the ecotype difference between Ophiopogonis Radix in Sichuan and Zhejiang province, and to provide a reference for the further study of the biological essence of geoauthentic medicinal materials. Method： Based on the traditional Chinese medicinal herbs of Ophiopogonis Radix, including 75 samples, the ecological difference between Ophiopogonis Radix in Sichuan and Zhejiang province was studied by molecular identification and ecological factors analysis. Result： It can be found that a stable variation existed at the 49 site in the psbA-trnH sequence of Ophiopogonis Radix in Sichuan and Zhejiang province, Ophiopogonis Radix in Sichuan as base adenine （A） and Ophiopogonis Radix in Zhejiang as base guanine （G） , this feature was both sequence difference. Ecological factor analysis showed that Sichuan and Zhejiang two regions had different geographical climate ecotype. Conclusion： There are obvious differences in genotype and ecological factors between Ophiopogonis Radix in Sichuan and Zhejiang province.

关 键 词：川麦冬 浙麦冬 DNA条形码 psbA-trnH序列 道地性 生态型差异 

分 类 号：R282[医药卫生—中药学] R93[医药卫生—中医学]