基于GC-MS分析蜜炙对甘草中挥发性成分的影响  

Influence of Mix-fring with Honey Technics on Volatile Components of Glycyrrhizae Radix et Rhizoma Based on GC-MS

作　　者：周倩[1,2] 王亮[1,2] 戴衍朋[2] 周洪雷[1] 

机构地区：[1]山东中医药大学,济南250355 [2]山东省中医药研究院,济南250014

出　　处：《中国实验方剂学杂志》2017年第17期87-90,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81603299);山东省自然科学基金项目(ZR2013HQ029);山东省重点研发项目(2016GSF202004)

摘　　要：目的:分析蜜炙对甘草中挥发性成分组成及相对质量分数的影响,为该炮制品的炮制机制研究提供参考。方法:采用水蒸气蒸馏法提取挥发油,利用气相色谱-质谱联用技术(GC-MS)对甘草不同样品中的挥发油成分进行确认,Restek Rxi-5ms毛细管柱(0.25 mm×30 m,0.25μm),程序升温(40℃,保持5 min,以7℃·min^(-1)升温至170℃,保持40 min,以2.0℃·min^(-1)升温至220℃,以5.0℃·min^(-1)升温至280℃),流量1.0 m L·min^(-1),载气为氦气;离子源为电子轰击电离源,分流比29∶1,电子能量70 e V,离子源温度200℃,接口温度280℃,溶剂延时3.5 min,检测器电压0.8 kV,灯丝发射电流750μA,倍增器电压1.2 kV。以面积归一化法计算各组分的相对质量分数。结果:生甘草中鉴定出36种成分,炙甘草中鉴定出20种成分。其中有4种为二者的共有组分,分别为乙苯、环己酮、邻苯二甲酸丁基2-戊基酯和6,6-二甲基富烯。甘草蜜炙后,4个共有组分的相对含量均有明显变化,分别由0.03%,0.12%,0.14%,0.12%提高到3.37%,13.24%,7.57%,10.84%。结论:甘草蜜炙后,其挥发性成分的组成和相对质量分数均发生非常显著的变化。Objective： To analyze the influence of mix-fring with honey technics on volatile components of Glycyrrhizae Radix et Rhizoma. Method： Steam distillation was used to extract the volatile oil from Glyeyrrhizae Radix et Rhizoma and GC-MS was used to analyze and identify these components, carrier gas was helium, temperature was rose by program, ionization mode was electron bombardment ion source. The relative contents of each component was calculated by area normalization method. Result： After analysis and identification, 36 components were identified in Glycyrrhizae Radix et Rhizoma, 20 components were identified in its processed product and there were 4 common components, including ethylbenzene, 6, 6-dimethylfenene, cyclohexanone and phthalic acid, butyl 2-pentyl ester. Before and after mix-fring with honey, the relative contents of 4 common components also had obvious changes from 0.03% , 0. 12% , 0. 12% , 0. 14% to 3.37% , 10.84% , 13.24% , 7.57% , respectively. Conclusion： The mix-fring with honey technics can change the compositions and contents of volatile components in Glycyrrhizae Radix et Rhizoma.

关 键 词：甘草 蜜炙 挥发油 乙苯 环己酮 邻苯二甲酸丁基2-戊基酯 6 6-二甲基富烯 

分 类 号：R283[医药卫生—中药学] R943.1[医药卫生—中医学]