检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
出 处:《中国实验方剂学杂志》2017年第17期138-143,共6页Chinese Journal of Experimental Traditional Medical Formulae
基 金:国家自然科学基金项目(81573771);江苏省自然科学基金项目(BK20151599)
摘 要:目的:探讨金丝桃苷(hyperoside,Hyp)对淀粉样蛋白(Aβ_(1-42))介导的血脑屏障(blood-brain barrier,BBB)体外模型损伤的影响。方法:本研究以Hyp干预纤维状Aβ_(1-42)介导的b End.3细胞与原代星形胶质细胞(astrocytes,As)BBB体外共培养模型的损伤,通过检测各培养组荧光素钠的通透性,比较各组BBB渗透性的变化,评价Hyp对Aβ_(1-42)介导的BBB的通透性的影响;采用噻唑蓝(MTT)比色法检测Hyp对Aβ_(1-42)介导的b End.3细胞损伤的细胞活力影响;再以蛋白免疫印迹法(Western blot)检测相关凋亡蛋白,初步探索其保护机制。结果:与模型组比较,Hyp低、中、高浓度组均能降低BBB的通透性(P<0.05,P<0.01);Hyp中、高浓度组能显著提高b End.3细胞活性(P<0.05);Western blot结果显示,经Hyp预处理的组别中,Bax/Bcl-2,cleaved半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)/Caspase-3的及细胞色素-C(Cyt-C)的表达量均明显下降,且差异均具有统计学意义(P<0.05)。结论:Hyp通过保护脑微血管内皮细胞抑制纤维状Aβ_(1-42)诱导的BBB通透性增加。Objective: To explore the effect of hyperoside (Hyp) on the damaged blood-brain barrier (BBB) induced by amyliod-beta protein (Aβ1-42) in vitro model. Method: In this experiment, Hyp intervention was used for the injury of fibrous Aβ1-42-mediated bEnd. 3 cells and primary astrocytes (astrocytes, As) BBB coculture models. By detecting the permeability of sodium fluorescein in various culture groups, BBB permeability changes were compared between various groups to evaluate the effect of Hyp on permeability of Aβ1-42-mediated BBB; methyl thiazolyl tetrazolium (MTT) was used to detect cells vitality and uncover the effect of Hyp on it; then in order to explore its protection mechanism, the apoptosis related proteins were detected by Western blot. Result: As compared with the model group, BBB permeability was reduced in Hyp low, middle and high dose groups (P 〈 0.05, P 〈 0.01 ) ; bEnd. 3 cells activity was improved in Hyp middle and high dose groups (P 〈 0.05) ; Western blot results showed that the ratio of Bax/Bcl-2, cleaved cysteine aspartic acid protease-3 (Caspase-3) /Caspase-3 and the expression levels of cytochrome-C (Cyt-C) were declined obviously in the Hyp groups, and the differences were statistically significant (P 〈 0.05). Conclusion: Hyp could inhibit the fibrous Aβ1-42induced permeability increase of BBB via protecting cerebral microvascular endothelial cells.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.117