白藜芦醇对兔膝骨关节炎的保护作用  

Protective properties of resveratrol on knee osteoarthritis in a rabbit model

作　　者：陈蓟 雷鸣[2] 周震全[3] 刘弼 肖德明[2] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院,518020 [2]北京大学深圳医院,518036 [3]南方医科大学深圳医院,518000

出　　处：《中国矫形外科杂志》2017年第17期1597-1601,共5页Orthopedic Journal of China

基　　金：国家自然科学基金资助项目(编号:81272032);深圳科技计划重点项目(编号:201101001)

摘　　要：[目的]研究白藜芦醇对兔膝骨性关节炎模型的保护作用。[方法]将40只成年新西兰兔(限雄性)作为实验兔,随机分为5组,正常组、模型组、低剂量白藜芦醇组、中剂量白藜芦醇组、高剂量白藜芦醇组,模型组及白藜芦醇组采用Hulth经典方法造骨关节炎模型。术后4周造模成功,各组分别干预,10周后行X线片检测,并取膝关节标本行HE染色、Mankins评分、micro CT扫描,数据作统计学处理。[结果]与模型组相比,白藜芦醇处理组的X线膝关节间隙改变和镜下软骨退变明显减轻。此外,白藜芦醇组的组织学Manking评分和micro CT的软骨下骨骨量体积比(BV/TV)及骨小梁数目(Tb.N)值也以剂量依赖的方式减低,各组间差异有统计学意义(P<0.05)。[结论]白藜芦醇能防止关节软骨退变,从而延缓骨关节炎进程。[Objective] To investigate the protective effect of resveratrol on knee osteoarthritis （OA） in a rabbit model. [Methods] Forty New Zealand rabbits （male） were randomly divided into 5 groups including normal control （group A） , model control （group B） , resveratrol intervention in low dosage （group C） , resveratrol intervention in middle dosage （group D） , as well as resveratrol intervention in high dosage （group E） . The OA was surgically induced using Huhh technique in groups B, C, D, and E. As soon as the OA models were confirmed to be successful at 4 weeks after surgery, a corre- sponding intervention was respectively given in each group. At 10 week after surgery, the intervention effect was evaluated using X-ray examination, histological observation and micro CT scanning. [Resultsl Compared with the model control groups, changes of the knee joint space in X-ray film, cartilage degeneration in histological observation was remarkably diminished in the resveratrol treated groups. Additionally, the Mankins score in histological evaluation, BV/TV and Tb.N values hy micro CT measurements were significantly decreased in the resveratro] treated groups in a dosage-dependent manner, associated with sta- tistical differences among the groups （P〈0.05） . [Conchlsion] Resveratrol can prevent degeneration of articular cartilage, thereby delay the progression of osteoartbritis.

关 键 词：白藜芦醇 骨关节炎 软骨 

分 类 号：R684.3[医药卫生—骨科学]