香青兰总黄酮对血管紧张素Ⅱ诱导血管平滑肌细胞黏附分子及基质金属蛋白酶表达的影响  

Inhibitory effects of Dracocephalum Moldavica L.total flavones on adhesion molecules and matrix metalloproteinases of rat vascular smooth muscle cells induced by AngⅡ

作　　者：曹文疆[1,2] 胡娜[2] 王新春[1] 郭新红[1] 袁勇[1] 程江[1,2] 

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子832008 [2]石河子大学医学院,新疆石河子832002

出　　处：《中国医院药学杂志》2017年第16期1566-1569,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金(编号:81460638;81660704;81360631)

摘　　要：目的:研究香青兰总黄酮对血管紧张素Ⅱ(AngⅡ)诱导的大鼠主动脉血管平滑肌细胞(VSMC)黏附分子及基质金属蛋白酶(MMPs)表达的影响。方法:采用贴壁法培养大鼠胸主动脉平滑肌细胞,以AngⅡ为诱导剂,建立VSMC细胞增殖的模型,分别应用10-7 mol·L^(-1) AngⅡ以及AngⅡ+不同浓度香青兰总黄酮组(25,50,100μg·mL^(-1))作用24 h,并设空白对照组进行比较。采用免疫组化法检测细胞中细胞间黏附分子(ICAM-1)、血管间黏附分子(VCAM-1)的表达水平。RT-PCR方法检测细胞MMP-2、MMP-9的mRNA表达水平。结果:与对照组比较,AngⅡ组能显著刺激大鼠VSMC细胞内ICAM-1、VCAM-1、MMP-2、MMP-9的表达,香青兰总黄酮不同剂量组联合AngⅡ可在一定程度上抑制AngⅡ诱导的VSMC细胞内ICAM-1、VCAM-1、MMP-2、MMP-9的表达,且呈现一定的剂量依赖关系趋势。结论:香青兰总黄酮具有抑制AngⅡ诱导VSMC黏附分子及基质金属蛋白酶表达的作用。OBJECTIVE To explore the possible mechanism of pharmacological activity of tilianin by observing the effects of Dracocephalum Moldavica L.total flavones（TFDM）on adhesion molecules and matrix metalloproteinases of rat vascular smooth muscle cells（VSMCs）induced by AngⅡ.METHODS VSMC were primary cultured by tissue sticking method.Cell proliferation model was established by stimulating with AngⅡ.Cell proliferation model was established by stimulating with10-7 mol·L^-1 AngⅡ.After AngⅡ stimulation,cells were treated with three dose of TFDM（25,50,100 mg·L^-1）for 24 hours,and a control group was assigned.The expression of intracellular cell adhesion molecules（ICAM-1）and vascular cell adhesion molecules（VCAM-1）were measured by immunohistochemistry.RT-PCR method was used to detect cell MMP-2 and MMP-9 mRNA expression levels.RESULTS Compared with control group,three dose groups of TFDM inhibited the expression of intracellular ICAM-1,VCAM-1,MMP-2 and MMP-9 of VSMCs induced by AngⅡin varying degrees in a dose dependent manner.CONCLUSION TFDM can inhibit the adhesion molecules and matrix metalloproteinases of VSMC induced by Ang Ⅱ.

关 键 词：香青兰总黄酮 血管紧张素Ⅱ 血管平滑肌细胞 ICAM-1 VCAM-1 MMP-2 MMP-9 

分 类 号：R285.5[医药卫生—中药学]