参慈胶囊联合顺铂对A549/DDP肺腺癌组织MDR1、MRP1、LRP表达的影响  

The Effect of Shenci Capsule Combined with Cisplatin on the Expression of MDR1,MRP1 and LRP in A549/DDP Lung Adenocarcinoma Tissues

作　　者：于礼建[1] 徐立群[1] 李志阳[2] 邬晓东[1] 梁昆[1] 韦伊儿 邹莹[1] 

机构地区：[1]广州医科大学附属肿瘤医院,广东广州510095 [2]广州医科大学肿瘤研究所

出　　处：《中国医学创新》2017年第24期1-4,共4页Medical Innovation of China

基　　金：2016年广东省中医药管理局课题(20162100);2014年广州市卫生局中医药和中西医结合科技项目(20142A010021)

摘　　要：目的:探讨参慈胶囊对人肺腺癌A549/DDP裸鼠体内多药耐药相关基因的影响。方法:将50只C57BL/6小鼠接种人肺腺癌细胞A549/DDP细胞悬液建立实体瘤模型,随机分为对照组、参慈胶囊组、参慈胶囊+顺铂组、顺铂组,各10只。对照组予等量生理盐水,其余各组荷瘤小鼠均用药21 d,断颈处死取肿瘤组织,采用FQ-PCR技术检测人肺腺癌A549/DDP肺癌组织MDR1、MRP1、LRP m RNA的表达情况。结果:MDR1 m RNA、MRP1 m RNA、LRP1 m RNA在各组均有表达,各治疗组的MDR1m RNA、MRP1 m RNA、LRP1 m RNA均低于对照组,差异均有统计学意义(P<0.01);参慈胶囊+顺铂组的MDR1 m RNA、MRP1 m RNA、LRP1 m RNA的表达均明显低于参慈胶囊组和顺铂组,差异均有统计学意义(P<0.01)。结论:通过降低MDR1、MRP1、LRP多药耐药相关基因的表达,可能是参慈胶囊逆转人肺腺癌A549/DDP裸鼠体内多药耐药的作用机制之一。Objective：To investigate the effect of Shenci Capsule on human lung adenocarcinoma A549/DDP nude mice multidrug-resistant genes in the body.Method：50 C57BL/6 mice were injected with human lung adenocarcinoma DDP-resistant cell sublines A549/DDP cell suspension to establish solid tumor models and randomly divided into control group,Shenci Capsule group,Shenci Capsule ＋ Cisplatin group,and Cisplatin group,10 mice in each group.The control group was treated with the same amount of normal saline,each group of the rest with tumor-burdened mice were treated with drugs.After 21 d all mice were sacrificed by broken neck and transplanted tumor tissues were cut off,the expression of MDR1,MRP and LRP m RNA in human lung adenocarcinoma A549/DDP tissue were detected by the FQ-PCR technique.Result：MDR1 mRNA,MRP1 m RNA,LRP1 mRNA were expressed in all groups,the levels of MDR1 mRNA,MRP1 mRNA,LRP1 mRNA in the treatment groups were lower than those in the control group,the differences were statistically significant（P〈0.01）;the expression of MDR1 mRNA,MRP1 mRNA,LRP1 mRNA in Shenci Capsule ＋ Cisplatin group was lower than those in Shenci Capsule group and Cisplatin group,the differences were statistically significant（P〈0.01）.Conclusion：By reducing the MDR1,MRP1 and LRP multi-resistant genes expression,which may is one of the mechanism of action Shenci Capsule reverse human lung adenocarcinoma DDP-resistant cell sublines A549/DDP nude mice multidrug-resistance in the body.

关 键 词：肺癌 参慈胶囊 顺铂 多药耐药基因1 人多药耐药相关蛋白1 肺耐药相关蛋白 

分 类 号：R734.2[医药卫生—肿瘤]