参芪复方对2型糖尿病大鼠腓肠肌病理形态及血清炎性因子的影响  

Effect of Shenqi Fufang(参芪复方) on Pathological Morphology and Serum Inflammatory Factors of Gastrocnemius in Type 2 Diabetic Rats

作　　者：钟文[1] 谢春光[1] 刘桠[1] 朱海燕[2] 高泓[1] 贾华楠[1] 

机构地区：[1]成都中医药大学临床医学院,四川省成都市十二桥路37号610075 [2]成都医学院第一附属医院

出　　处：《中医杂志》2017年第17期1495-1498,共4页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81503566)

摘　　要：目的探讨参芪复方对2型糖尿病骨骼肌损害的影响及可能作用机制。方法 18只自发性2型糖尿病(GK)大鼠随机分为模型组、参芪复方组及西格列汀组,每组6只;另设Wistar大鼠6只为正常对照组。除正常对照组外,其余各组给予高脂饮食连续8周建立2型糖尿病模型。造模同时正常对照组、模型组均按5 ml/(kg·d)给予生理盐水灌胃,参芪复方组按1.44 g/(kg·d)给予参芪复方浸膏灌胃,西格列汀组按16 mg/(kg·d)给予磷酸西格列汀片混悬液灌胃。试验结束后检测各组大鼠血清胰岛素样生长因子1(IGF-1)、肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)含量,取腓肠肌称取湿重并观察其病理形态学改变,检测腓肠肌p70s6k1蛋白表达。结果与正常对照组比较,模型组大鼠腓肠肌湿重、血清IGF-1水平及p70s6k1蛋白明显降低(P<0.05),血清TNF-α、IL-1β含量明显升高(P<0.05),且病理观察发现腓肠肌细胞萎缩,有较大面积水肿,肌间淋巴细胞浸润。与模型组比较,西格列汀组及参芪复方组大鼠腓肠肌湿重、IGF-1含量及p70s6k蛋白表达均明显升高,TNF-α、IL-1β含量明显降低(P<0.05),且病理观察未见明显肌细胞萎缩、水肿,炎细胞浸润少。西格列汀组与参芪复方组各指标比较,差异均无统计学意义(P>0.05)。结论参芪复方可减轻糖尿病骨骼肌损害,其可能机制是减少炎性因子含量,升高血清IGF-1水平,从而维持骨骼肌质量。Objective To explore the effect of Shenqi Fufang（ 参芪复方,SF） on skeletal muscle damage of type2 diabetes mellitus（ T2DM） and its possible mechanism. Methods Eighteen rats with spontaneous type 2 diabetes mellitus（ GK） were randomized into the model group,SF group and sitagliptin group with six rats in each group,and six more Wistar rats were selected as the control group. Each group was given high fat diet for 8 weeks to build T2DM model except for the control group. At the same time,the control group and the model group were given normal saline5 ml/（ kg·d） by gastric perfusion,the SF group was given SF extract 1. 44 g/（ kg ·d） by gastric perfusion,and sitagliptin group was given sitagliptin phosphate suspension 16 ml/（ kg·d） by gastric perfusion. Eight weeks later,the levels of serum insulin-like growth factor 1（ IGF-1）,tumor necrosis factor-α（ TNF-α） and interleukin-1β（ IL-1β）were measured in each group,the gastrocnemius muscle was taken out to get the wet weight and observe the pathological changes,and the expression of p70s6k1 protein was detected. Results Compared with the control group,the wet weight of the gastrocnemius muscle,the serum IGF-1 level and p70s6k1 protein expression in the model group significantly decreased（ P〈0. 05）,while the levels of serum TNF-α and IL-1β significantly increased（ P〈0. 05）.Through pathological observation,there existed gastrocnemius muscle cells atrophy,a larger area of edema and interstitial lymphocyte infiltration. Compared with the model group,the wet weight of the gastrocnemius muscle,IGF-1content and p70s6 k protein expression in the sitagliptin group and the SF group significantly increased,the contents of TNF-α and IL-1β significantly decreased（ P〈0. 05）,and there were no obvious muscle cell atrophy and edema,and also little inflammatory cell infiltration. There was no significant difference between the sitagliptin group and SF group（ P〈0. 05）. Conclusion SF could reduce the diabetic skeleta

关 键 词：2型糖尿病 骨骼肌损害 参芪复方 炎性细胞因子 胰岛素样生长因子1 

分 类 号：R285.5[医药卫生—中药学]