藏药渣驯正丁醇部位对刀豆蛋白A致小鼠肝损伤的保护作用  

Protective Effect of n-Butanol Fraction from Tibetan Medicine Brag-zhun on Liver Injury in Mice Induced by Concanavalin A

作　　者：王文倩[1] 叶翠萍[1] 曾勇[1] 钟世红[2] 古锐[1] 让坚 

机构地区：[1]成都中医药大学,四川成都611137 [2]成都医学院,四川成都610081 [3]阿坝州藏医院,四川马尔康624000

出　　处：《中药材》2016年第12期2846-2849,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然基金面上项目(313911)

摘　　要：目的:研究藏药渣驯正丁醇部位对刀豆蛋白A(Con A)所致肝损伤的保护作用,并初步探讨其作用机制。方法:小鼠尾静脉注射Con A建立肝损伤模型,测定血清ALT、AST、TNF-α、IFN-γ及肝组织SOD、MDA、Caspase-3、Caspase-8水平,HE染色法观察肝组织病理学变化。结果:渣驯正丁醇部位能显著降低Con A所致的肝损伤小鼠血清ALT、AST、TNF-α、IFN-γ及肝组织MDA含量,升高肝组织SOD活性,降低肝组织Caspase-3、Caspase-8活化水平,减轻肝组织病理学损伤。结论:藏药渣驯正丁醇部位对Con A致小鼠肝损伤有一定的保护作用,其机制可能与抑制炎症因子释放、抗氧化活性、抗细胞凋亡有关。Objective： To investigate the protective effect and mechanism of n-butanol fraction from Tibetan medicine Brag-zhun on liver injury in mice which induced by Concanavalin A. Methods ：The experimental mice were injected with Concanavalin A via the tail vein to establish the liver injury model. Serum ALT, AST, TNF-α and IFN-γ content were detected. Liver tissue SOD, MDA, Caspase-3 and Caspase-8 activities were measured. The pathological changes were detected by HE staining. Results ：n-Butanol fraction from Tibet- an medicine Brag-zhun reduced the activities of ALT, AST, TNF-α, IFN-γ in serum, and hepatic MDA content, the Caspase-3 and Caspase-8 activities of liver tissues were decreased, hepatic SOD activity were increased, and alleviated the pathological injury of liver tissue which induced by Concanavalin A. Conclusion ： n-Butanol fraction from Tibetan medicine Brag-zhun shows the protective effect on liver injury, which may be related to the inhibiting the release of inflammatory factors ability, antioxidative ability and anti-liver apopto- sis.

关 键 词：渣驯正丁醇部位 刀豆蛋白A 肝损伤 

分 类 号：R285.5[医药卫生—中药学]