人腰椎小关节软骨不同染色方法的差异比较  

Different staining methods used for human lumbar facet joint cartilage:a comparative study

作　　者：黄磊涛 赖琦[2] 李帆[2] 毕海迪 吴霞[3] 刘序强[2] 张斌[2] 戴闽[2] 

机构地区：[1]南昌大学,江西省南昌市330006 [2]南昌大学第一附属医院骨科,江西省人工关节工程技术研究中心,江西省南昌市330006 [3]南昌大学第一附属医院心胸外科,江西省南昌市330006

出　　处：《中国组织工程研究》2017年第24期3784-3789,共6页Chinese Journal of Tissue Engineering Research

基　　金：南昌大学研究生创新专项资金(cx2016390)~~

摘　　要：背景:随着现代病理技术的不断创新及发展,在疾病诊断方面显著减少其误诊率,但特殊染色法依然是病理诊断的最主要手段。目的:比较不同的特殊染色方法显示人腰椎小关节结构的优缺点。方法:收集腰椎手术患者的L4/5小关节软骨标本,行苏木精-伊红染色、番红O染色、甲苯胺蓝染色、Masson染色、番红-固绿染色,观察软骨结构。结果与结论:苏木精-伊红染色、番红-固绿染色和甲苯胺蓝染色均可清晰观察到关节软骨各层结构。其中苏木精-伊红染色能较清楚显示软骨层、潮线及软骨下骨,软骨细胞核呈蓝紫色。番红-固绿染色清晰显示软骨4层结构:浅层(软骨表面),中间层(细胞圆形,着色较淡,排列规律),柱状细胞层(细胞较大,多核,排列整齐),显示软骨基质呈均匀浅红,软骨下骨呈绿色,软骨组织与骨组织分对比鲜明。甲苯胺蓝染色显示组织结构层欠清,胞核染色清晰,胞浆几乎不着色,基质呈淡蓝紫色。而番红O染色软骨层及软骨下骨层分界比较清晰,软骨层呈均匀深红色,细胞染色较淡,与软骨基质分界不明显。Masson染色对胶原纤维较为清晰,但只能大致显示软骨与软骨下骨结构。结果说明番红-固绿染色显示软骨各层和软骨下骨结构最佳;苏木精-伊红染色观察软骨细胞形态变化较其他方法清楚,Masson染色最次。BACKGROUND： With the development of modern pathological techniques, the misdiagnosis rate has been reduced remarkably, but special stains are still the most important method for pathological diagnosis. OBJECTIVE： To compare the advantages and disadvantages of different special stains used for observing the structure of human lumbar facet joints. METHODS： The specimens of facet joint cartilage at L1/5 level were collected from patients undergoing lumbar surgery, and then stained with hematoxylin-eosin, safranin O, toluidine blue, Masson, and saranin-O-fast green for structure observation. RESULTS AND CONCLUSION： The structure of the articular cartilage could be observed clearly through hematoxylin-eosin, toluidine blue, and saranin-O-fast green staining. The cartilage surface, tidemark, and subchondral bone were shown by the hematoxylin-eosin staining, with the presence of violet chondrocyte nuclei. Safranin-O-fast green staining showed the four layers of the cartilage cleady, including the shallow layer （cartilage surface）, middle layer （spherical cells arranged in disorder）, columnar cell layer （large and multinucleated chondrocytes arranged neatly）, tidemark, subchondral bone layer; and the cartilage matrix was reddish uniformly, the subchondral bone was green, and the cartilage and bone tissue showed a striking contrast. The cartilage structure was unclear in toluidine blue staining, with clear nuclei and almost no coloring cytoplasm, but the matrix appeared with slight purplish blue. Safranin O staining showed that the cartilage was red, which had no obvious boundary with the cartilage matrix, and chondrocytes were stained lightly. Masson staining showed clear collagen fibers, but the structures of the cartilage and subchondral were obscure. To conclude, safranin-O-fast green staining can achieve the best results, followed by hematoxylin-eosin staining and Masson staining in turn.

关 键 词：腰椎 染色与标记 组织工程 组织构建 软骨组织工程 腰椎小关节 关节软骨 特殊染色 苏木精-伊红染色 番红O染色 甲苯胺蓝染色 MASSON染色 番红-固绿染色 

分 类 号：R318[医药卫生—生物医学工程]