雌激素缺乏状态下牙周组织中核转录因子κB及白细胞介素17的表达变化  

Effect of estrogen deficiency on the expression levels of nuclear factor-kappa B and interleukin-17 in periodontal tissues

作　　者：贾智[1] 张丰伟[1] 祁星颖 闫潇[1] 华烨[1] 赵梦明[1] 刘大勇[1] 

机构地区：[1]天津医科大学口腔医院牙体牙髓科,天津市300070

出　　处：《中国组织工程研究》2017年第24期3796-3802,共7页Chinese Journal of Tissue Engineering Research

基　　金：天津市自然科学基金一般项目(15JCYBJC50200)和重点项目(16JCZDJC32800)~~

摘　　要：背景:绝经期后骨质疏松症与牙周组织疾病有着十分密切的关系,而其作用机制尚不清楚。目的:通过切除卵巢建立雌激素缺乏状态下骨质疏松小鼠模型,观察在此状态下牙槽骨组织中核转录因子κB以及血清与牙龈组织中白细胞介素17的表达变化。方法:将3月龄雌性C3H小鼠随机分为卵巢切除组及假手术组。术后10周,苏木精-伊红染色观察下颌骨及股骨组织学改变;RT-PCR检测下颌骨中成骨相关因子OCN及Runx2表达变化;免疫组化染色及Western Blot检测下颌骨中核转录因子κB表达;CBA流式液相多重蛋白定量技术检测血清及牙龈组织匀浆中白细胞介素17水平。结果与结论:(1)卵巢切除组小鼠股骨的骨皮质变薄,骨髓腔扩大,骨小梁变细、数目减少、密度降低、结构不完整,骨吸收陷窝样结构增多;(2)卵巢切除组牙槽骨组织中OCN及Runx2相对表达量较假手术组低;(3)卵巢切除组小鼠下颌骨中核转录因子κB活化形式P65蛋白呈阳性表达;(4)卵巢切除组牙槽骨组织中核转录因子κB活化形式P65相对表达量高于假手术组;(5)卵巢切除组小鼠血清中白细胞介素17表达水平高于假手术组,但牙龈组织中白细胞介素17表达水平与假手术组相比无明显变化;(6)实验表明,雌激素缺乏导致的核转录因子κB信号通路激活可能在牙周骨吸收中发挥一定作用;而牙周局部组织内的白细胞介素17可能并非此状态下牙周组织破坏的关键因子。BACKGROUND： There is a very close relationship between osteoporosis and periodontal disease in postmenopausal women, but the mechanism remains unclear. OBJECTIVE： To investigate the expression of nuclear factor-KB in the alveolar bone and intedeukin-17 in the serum and gingiva in the mouse model of osteoporosis caused by ovariectomy. METHODS： Female mice aged 3 months were randomly divided into ovariectomy and sham operation groups. At 6 months after surgery, the mouse models were evaluated histologically on the submandibular bone and thigh bone stained with hematoxylin and eosin. In the submandibular bone, the expression levels of OCN and Runx2 were detected by RT-PCR, and the expression level of nuclear factor-KB was detected by immunohistochemical staining and western blot assay. Besides, the expression level of interleukin-17 in the serum and gingival homogenate was evaluated using Cytometric Beads Array. RESULTS AND CONCLUSION： The thigh bone in the ovadectomy group revealed the thin cortical bone, enlarged marrow cavity, and increased resorption lacunae, as well as fewer, thinner trabeculae with lower density and irregular structure. Compared with the sham operation group, the expression levels of OCN and Runx2 in the alveolar bone were decreased in the ovariectomy group. The activation of nuclear factor-KB （P65） appeared with P65 positive expression in the submandibular bone in the ovariectomy group, and the relative expression level was higher than that in the sham operation group. The serum level of interleukin-17 in the ovariectomy group was higher than that in the sham operation group, but the level in the gingival tissue showed no significant difference between the two groups. These results indicate that estrogen deficiency after ovadectomy can activate nuclear factor-KB signal pathway to play a role in periodontal osteolysis. However interleukin-17 in the local periodontal tissue may not be a key cytokine to damage the periodontal tissue.

关 键 词：骨质疏松 绝经后 卵巢切除术 NF-κB 白细胞介素17 组织工程 组织构建 卵巢切除 雌激素 骨代谢 牙周组织 核转录因子ΚB 天津市自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]