葛根素对支气管上皮细胞和嗜中性粒细胞共培养体系基质金属蛋白酶组织抑制剂-2表达的影响  

Effect of puerarin on the expression of TIMP-2 in co-cultured bronchial epithelial cells and neutrophils

作　　者：庞伟[1] 王成彬[2] PANG Wei WANG Cheng-bin(Department of Clinical laboratory, Affiliated Hospital of Logistics University of PAP, Tianjin 300162,China)

机构地区：[1]武警后勤学院附属医院检验科,天津300162 [2]解放军总医院临检科,北京100853

出　　处：《武警后勤学院学报（医学版）》2017年第6期461-465,共5页Journal of Logistics University of PAP（Medical Sciences）

基　　金：国家自然科学基金项目(30873419);武警后勤学院院级课题项目(FYM201608)

摘　　要：【目的】观察支气管上皮细胞(BEAS-2B细胞)和嗜中性粒细胞(Neutrophils细胞)接触共培养体系中基质金属蛋白酶组织抑制剂-2(tissue inhibitors of metalloproteinases-2,TIMP-2)合成,探讨不同剂量葛根素对共培养体系TIMP-2的抑制作用。【方法】应用蛋白芯片筛查不同培养体系炎性细胞因子表达;应用ELISA方法定量测量TIMP-2浓度;应用Real-time PCR方法检测TIMP-2基因表达。【结果】BEAS-2B细胞和Neutrophils细胞联合培养上清液中TIMP-2浓度升高,基因表达水平明显上调(P<0.05)。经葛根素干预可显著抑制两种细胞联合培养TIMP-2浓度及基因表达(P<0.05)。【结论】Neutrophils细胞与BEAS-2B细胞联合培养可显著增加细胞培养上清液中炎性细胞因子TIMP-2浓度,上调BEAS-2B细胞中TIMP-2基因表达;葛根素可下调TIMP-2浓度及表达。【Objective】To observe the expression of TIMP-2 in co-cultured BEAS-2B cells and neutrophils, and explore the inhibitory effect of different doses of puerarin on TIMP-2 in the co-cultured system.【Methods】The expression of inflammatory cytokines in different culture systems was detected by protein chip technology. The concentrations of TIMP-2 were quantified by enzyme linked immunosorbent assay（ELISA）. The gene expression of TIMP-2 was determined by real-time quantitative polymerase chain reaction（Real-time q PCR）.【Results】The concentration of TIMP-2 in the supernatant of co-cultured BEAS-2B cells and neutrophils increased（P〈0.05）, and the gene expression of TIMP-2 was significantly up-regulated（P〈0.05）. Puerarin could significantly decrease the concentration of TIMP-2 and obviously downregulate the gene expression of TIMP-2 in co-cultured BEAS-2B cells and neutrophils（P〈0.05）.【Conclusion】In the co-cultured neutrophils and BEAS-2B cells, the concentration of TIMP-2 can significantly increase.The gene expression of TIMP-2 can be upregulated in BEAS-2B cells. Puerarin can decrease the concentration of TIMP-2 and downregulate its expression.

关 键 词：支气管上皮细胞 抑制 基质金属蛋白酶组织抑制剂-2 

分 类 号：R285.5[医药卫生—中药学]