鬼臼毒素衍生物ZM-10对口腔鳞状细胞癌KB细胞株的作用及其机制研究  

Effect of a podophyllotoxin derivative ZM-10 on oral cavity squamous cell carcinoma(KB)cell line and its mechanism

作　　者：高雅婷 曹诗 彭悦[2] 王辉[3] 王毅铮[2] 陈虹[2] GAO Ya-ting CAO Shi PENG Yue WANG Hui WANG Yi-zheng CHEN Hong(Nanchang Detachment of Jiangx Armed Corps, Nanchang 330038, Chin)

机构地区：[1]武警江西总队南昌支队,江西南昌330038 [2]武警后勤学院天津市职业与环境危害防制重点实验室,天津300162 [3]河北医科大学第一医院骨科,河北石家庄050000

出　　处：《武警后勤学院学报（医学版）》2017年第6期466-469,共4页Journal of Logistics University of PAP（Medical Sciences）

摘　　要：【目的】对鬼臼毒素进行结构改造得到新衍生物ZM-10,观察其对口腔鳞状细胞癌KB细胞株的作用,并探讨其作用机制。【方法】MTT法和流式细胞术检测鬼臼毒素衍生物ZM-10对KB细胞增殖作用的影响;荧光染色实验检测鬼臼毒素衍生物ZM-10作用后KB细胞形态学变化情况;RT-PCR法检测鬼臼毒素衍生物ZM-10作用KB细胞后P53、Caspase-3、Bcl-2以及Bax等相关基因表达的变化情况。【结果】MTT结果显示鬼臼毒素衍生物ZM-10对口腔鳞状细胞癌KB细胞株的半数抑制浓度为3μmol/L。流式细胞检测结果显示鬼臼毒素衍生物ZM-10对KB细胞增殖的各个时相均有抑制作用,且使细胞周期阻断在G2+M期。RT-PCR结果显示鬼臼毒素衍生物ZM-10作用后,细胞中P53、Caspase-3和Bax的m RNA的表达量均显著增加(P<0.05),而Bcl-2的m RNA的表达明显下调(P<0.05)。【结论】鬼臼毒素新衍生物ZM-10通过诱导KB细胞发生凋亡而抑制细胞增殖。【Objective】To modify the structure of podophyllotoxin to get a new derivative ZM-10, observe its effect on oral cavity squamous cell carcinoma（KB） cell line, and explore the mechanism.【Methods】The effect of ZM-10 on the proliferation of KB cells was detected by MTT assay and flow cytometry. The morphological changes of KB cells were tested by fluorescence staining. RT-PCR was used to detect the gene expression changes of P53, Caspase-3, Bcl-2 and Bax in KB cells.【Results】The results of MTT assay showed that IC50 of ZM-10 on KB cells was 3μmol/L. The results of flow cytometric analysis indicated that ZM-10 inhibited the proliferation of KB cells at various phases and induced an accumu1 ation in G2＋M phase of cell cycle. The RT-PCR results showed that the m RNA expression levels of P53, Caspase-3 and Bax significantly increased after ZM-10 treatment（P〈0.05）, while the m RNA expression level of Bcl-2 obviously decreased（P〈0.05）.【Conclusion】The new derivative of podophyllotoxin ZM-10 inhibits the cell proliferation by inducing the apoptosis in KB cells.

关 键 词：鬼臼毒素 衍生物 KB细胞 凋亡 

分 类 号：R34[医药卫生—基础医学]