华东地区献血者人群隐匿性乙型肝炎病毒感染及S区变异分析  

Study on prevalence and mutation of amino acid sequence in S gene of OBI blood donors in three blood centers in China

作　　者：蒋菲菲[1] 吕蓉[1] 李敏[2] 赵阳[1] 林洪铿[3] 钱榕[4] 

机构地区：[1]安徽省血液中心,安徽合肥230031 [2]安徽医科大学第四附属医院 [3]福建省血液中心 [4]江西省血液中心

出　　处：《中国输血杂志》2017年第7期762-766,共5页Chinese Journal of Blood Transfusion

摘　　要：目的了解安徽、福建、江西三地血液中心无偿献血者隐匿性乙型肝炎病毒(OBI)感染情况,探讨HBV基因分型特征及S区氨基酸变异特点。方法对2013年1月-2014年5月安徽省血液中心、2013年6月-2013年11月、2014年3月-2014年12月福建省血液中心、2013年6月-2013年9月江西省血液中心乙肝表面抗原酶联免疫法检测阴性(HBs Ag-)、核酸HBV鉴别试验阳性(NAT+)的102例无偿献血者标本进行抗-HBc检测,同时应用巢式PCR方法对这些标本进行HBV S区扩增并测序,采用MEGA 6软件进行HBV基因分型及S区氨基酸突变分析。结果在安徽省血液中心123 046例无偿献血者中筛查出21例HBs Ag-NAT+无偿献血者标本,OBI感染率为0.017%(21/123 046),其中76.2%(16/21)为抗-HBc阳性,这21例标本中,15例扩增出HBV S区片段,8例B型,7例C型;福建省血液中心HBs Ag-NAT+无偿献血者标本共51例,76.5%(39/51)为抗-HBc阳性,其中16例扩增出HBV S区片段,14例B型,2例C型;江西省血液中心HBs Ag-NAT+献血者标本共30例,80%(24/30)为抗-HBc阳性,其中4例扩增出HBV S区片段,1例B型,3例C型。35例扩增产物的OBI标本中,74.3%(26/35)出现HBV S基因MHR区氨基酸置换突变,主要集中在乙型肝炎病毒"α"决定簇区域。结论安徽地区无偿献血者OBI感染率为0.017%,福建及江西地区也有一定OBI感染;成功扩增HBV S区的OBI标本中,抗-HBc阳性率为安徽73.3%、福建93.8%、江西100%;B型为主要HBV基因型;HBV S基因MHR区变异,尤其是HBs Ag"α"决定簇区置换突变,可能是OBI形成原因之一。Objective To study the prevalence of the occuh hepatitis B virus infection （OBI） and the mutation of ami- no acid sequence in S gene of voluntary blood donors in AnHui/FuJian/Jiang Xi Province Blood centers. Methods Serologic testing for anti-HBc by ELISA was performed with HBsAg-HBV DNA＋ samples from voluntary blood donors in three province blood centers. The S region of HBV of those samples was amplified and sequenced. The genotype and mutation of amino acid sequence were analyzed by MEGA6. Results 2i in 123046 blood donors from AnHui Province blood center were HBsAg- HBV DNA＋, the prevalence of OBI was 0. 017% ,and 76. 2% of these OBI samples was positive in anti-HBc, S region was amplified by nest-PCR in 15 OBI samples, 8 of them were B genotype, the others were C genotype. 39 samples of 51 OBI blood donors from FuJian Province blood center were anti-HBc positive, 16 samples of those OBI donors were amplified S re- gion, 14 were B genotype, the others were C genotype. There are 30 OBI blood donors from JiangXi Province blood center, 24 of them were anti-HBc positive, S region was amplified in 4 samples, 1 was B genotype, the others were C genotype. Of all 35 OBI samples, 26 showed amino acid mutation, which was in MHR region of S gene, especially in HBV ＂a＂ epitope. Conclusion The rate of prevalence of OBI in AnHui Province was 0. 017%, there was also certain OBI infection in FuJian and JiangXi Province. In the OBI samples which were amplified S region, the positive rates of anti-HBc in three blood centers were 73.3% ,93.8%, 100%. B Genotype was the main HBV genotype. The mutation in MHR region of S gene, especially in HBV ＂a＂ epitope, may be one of the reasons to cause OBI.

关 键 词：献血者 隐匿性乙型肝炎病毒感染(OBI) S区 变异 

分 类 号：R193.3[医药卫生—卫生事业管理] R512.62[医药卫生—公共卫生与预防医学]