改良白膜法汇集浓缩少白细胞血小板质量的研究  

Study on the quality of concentration leukocyte-poor platelet concentrates by improved buffy coat

作　　者：杜晓明 陈安心 陈丽萍 孔福珠 钱江 何星 庄顺宏 

机构地区：[1]金华市中心血站,浙江金华321000 [2]金华市中心医院

出　　处：《中国输血杂志》2017年第7期829-831,共3页Chinese Journal of Blood Transfusion

基　　金：金华市科学技术研究计划项目(项目编号:2010-3-072)

摘　　要：目的研究改良白膜法汇集浓缩少白细胞血小板质量,为今后开展汇集浓缩血小板的制备提供依据。方法采用改良白膜法制备汇集浓缩血小板56袋检测各项质量指标,并随机抽取56袋单采血小板样本作比对;将37袋汇集浓缩血小板于贮存期d1(即采集当天)、d2、d3分别取样,用白细胞滤器进行过滤,测定不同贮存时间、过滤前后血小板膜表面糖蛋白分子CD62P和PAC-1的表达率;随机抽取37袋单采血小板作比对。结果改良白膜法制备的汇集浓缩少白细胞血小板容积、血小板含量、Ph值、红细胞混入量等检测结果与单采血小板各质量指标间比较差异无统计学意义(P>0.05),其白细胞混入量远低于单采血小板中白细胞混入量两者间存在显著性差异(P<0.01),无菌试验均为阴性;汇集浓缩血小板贮存期3d内滤白前后血小板膜表面糖蛋白分子CD62P和PAC-1的表达率均无统计学意义(P>0.05);贮存期3d内汇集浓缩血小板及单采血小板膜表面糖蛋白分子CD62P和PAC-1的表达率也均无统计学意义(P>0.05)。结论改良白膜法汇集浓缩少白细胞血小板各项质量指标均达到混合浓缩血小板及单采血小板国家标准,血小板体外活性保持良好;血小板专用型白细胞滤器对贮存期3d内的汇集浓缩血小板进行滤白处理不会造成血小板的明显活化和损伤;血小板振荡仪(22±2)℃振荡保存3d的汇集浓缩(单采)血小板存在持续的活化损伤,但活化和损伤并未明显改变血小板的体外活性。Objective To investigate the quality of concentrated platelet with less WBC by improved white membrane method, so as to provide the basis for preparation of the concentrated platelet in the future. Methods Testing the quality indicators of 56 cases of concentrated platelet with less white blood cells by improved white membrane method, and comparing the quality indicators of 56 cases of collected platelet at random by machine.Testing the expression rate of platelet membrane surface glycoprotein molecules CD62P and PAC-1 before and after filtering in different storage time, and comparing the ex- pression rate of 37 cases of collected platelets at random by machine. Results The differences of quality indicators between concentrated platelet with less white blood cells by improved white membrane methods and collected platelet at random by machine were not statistically significant （P〉0. 05）, such as volume, platelet content, Ph, amount of RBC mixed within.But The differences of amount of WBC mixed with were statistically significant （P〈 0. 01 ）, both of sterility tests were negative. The differences of the expression rate of platelet membrane surface glycoprotein molecules CD62P and PAC-1 during storage period within 3 days were not statistically significant （P〉0. 05）. Conclusion The various quality indicators of concentrated platelet with less white blood cells by improved white membrane methods attain mixed concentrated platelet and platelet na- tional standards,and in vitro platelet activity kept well.Platelet special leucocyte filter does not cause obvious platelet activa- tion and damage within three days of storage period of concentrated platelet in filter processing.Collected platelet by machine is being activated and damaged continuously when kept in platelet oscillation device 22±2℃ for 3 days, but activation and damage does not significantly change the platelet activity in vitro.

关 键 词：汇集血小板 改良白膜法 流式细胞仪 血小板活化 

分 类 号：R457.14[医药卫生—治疗学] R331.14[医药卫生—临床医学]