机构地区:[1]川北医学院医学检验系,南充637000 [2]川北医学院附属医院检验科
出 处:《中华风湿病学杂志》2017年第8期542-546,I0001,共6页Chinese Journal of Rheumatology
摘 要:目的比较SLE患者外周血中性粒细胞胞外诱捕网(NETs)形成的水平在SLE患者与健康对照组之间的差异,并分析其与SLE临床指标之间的相关性。方法收集44例女性SLE患者(活动期21例,稳定期23例),同时收集年龄相匹配的女性健康体检者42名作为健康对照。采用荧光光度法检测外周血中性粒细胞的NETs荧光强度,ELISA法检测血浆中中性粒细胞弹性蛋白酶(NE)浓度,比较二者在SLE组与对照组之间、SLE活动期组与SLE稳定期组之间的差异,并分析二者与SLE患者ESR、SLEDAI、抗dsDNA抗体等指标的相关性,组间比较采用方差分析和t检验,相关性分析采用Spearman秩检验。并采用细胞免疫荧光法结合激光共聚焦显微镜观察NETs形态。结果①女性SLE患者组NETs荧光强度显著高于健康对照组[(241±139)和(173±135),t=2.31,P〈0.05];SLE活动期组与稳定期组NETs荧光强度为[(274±168)和(211±102),t=1.52,P〉0.05],差异无统计学意义。② SLE组与健康对照组比较,血浆中NE浓度为[(102±47)和(62±22),t=4.38,P〈0.01],差异有统计学意义;但SLE活动期组与稳定期组NE浓度[(104±43)和(96±48),t=0.50,P〉0.05],差异无统计学意义。③ SLE患者NETs荧光强度和血浆中NE浓度均与SLEDAI呈正相关,与ESR、抗dsDNA抗体等差异均无统计学意义。④激光共聚焦显微镜下观察到SLE患者较健康对照NETs形成增强。 结论SLE患者外周血中性粒细胞NETs的形成增强,并可能与SLE的活动程度有关,但未发现其与自身抗体的产生有关。ObjectiveTo assay the level of neutrophil extracellular traps (NETs) in systemic lupus erythematosus (SLE) patients and healthy controls and compare the difference between SLE group and control group, and to analyze between the level of NETs and related laboratory parameters.MethodsForty-four females patients with SLE were recruited as research subjects, with 24 cases in the active stage and 20 in stable stage. Forty-two healthy female volunteers matched in age were enrolled as control subjects. The fluorescence intensity of NETs in neutrophils was detected by fluorospectrophotometry. The concentration of neutrophil elastase in plasma was quantitatively detected. Statistical analysis of the difference of level of NETs between the SLE group and control group was conducted. Then the correlation between the fluorescence intensity of NETs and erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), and anti-double-stranded DNA (dsDNA), and anti-histone, and anti-nucleosome, and systemic lupus erythematosus disease activity index (SLEDAI) was analyzed respectively. The same tests were conducted for the level of NE. The results of the two groups were compared using analysis of variance and the relevance was analyzed using Spearman correlation analysis. NETs morphosis was observated by immunoflurescence method, and the difference of NETs between SLE group and control group was analyzed.Results① The fluorescence intensity of NETs was significantly increased in SLE group(241±139) than that in the control group (173±135), (t=2.31, P〈0.05). ② The concentration of NE in the SLE group (102±47) was significantly higher than that in the control group (62±22), t= 4.38, P〈0.01. No difference of NETs [(274±168) vs (211±102), t=1.52, P〉0.05] and NE concentration [(104±43) vs (96±48), t=0.50, P〉0.05] between SLE active stage and stable stage was detected. ③ The fluorescence intensity of NETs in SLE patients was positively correlated with SLED
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