PAR4、TRPV1在电针缓解肠易激综合征大鼠内脏痛敏中的作用研究  

Involvement of PAR4 and TRPV1 in Electroacupuncture on Visceral Hypersensitivity in IBS Rats

作　　者：张亚楠[1] 王媛[1] 赵海军[1] 韩晓春[1] 夏雷[1] 季旭明[1] 王世军[1] 

机构地区：[1]山东中医药大学中医学院,山东济南250355

出　　处：《中华中医药学刊》2017年第9期2268-2271,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB505206);山东省自然科学基金项目(ZR2014HQ032)

摘　　要：目的:观察PAR4、TRPV1在电针缓解肠易激综合征(irritable bowel syndrome,IBS)内脏痛敏中的作用,探讨针刺缓解大鼠内脏痛敏的可能机制。方法:将SPF级成年雄性Wistar大鼠32只随机分为正常对照组、模型组、电针大肠俞组、电针上巨虚组,每组8只,采用乙酸灌肠法复制IBS内脏痛敏模型。造模成功后电针治疗,每天1次,连续7 d。在电针结束后,观察各组大鼠粪便性状并根据粪便性状分级进行粪便性状的评分,检测粪便含水量和引起腹部回缩反射的最小容量阈值,采用western-blotting检测大鼠结肠组织中PAR4、TRPV1蛋白的含量。结果:模型组大鼠粪便性状评分和粪便含水量与正常对照组相比明显增加(P<0.01,P<0.01),电针大肠俞组、电针上巨虚组大鼠粪便性状评分(P<0.01,P<0.01)和粪便含水量降低(P<0.05,P<0.05)。模型组与正常对照组相比引起大鼠腹部回缩反射的容量阈值明显降低(P<0.01),电针大肠俞和电针上巨虚后引起大鼠腹部回缩反射的容量阈值显著增加(P<0.01,P<0.01);模型组结肠组织PAR4蛋白的表达水平与正常对照组相比有升高的趋势,但差异无统计学意义(P>0.05);与模型组相比电针上巨虚组结肠组织PAR4蛋白表达水平下降,差异有统计学意义(P<0.05)。模型组结肠组织TRPV1蛋白的表达水平与正常对照组相比升高(P<0.05)。与模型组相比电针大肠俞组、电针上巨虚组结肠组织TRPV1蛋白表达水平有下降的趋势。结论:电针可以有效缓解IBS内脏痛敏,其机制可能和调控IBS内脏痛敏大鼠结肠PAR4、TRPV1蛋白的表达有关。Objective： To observe effects of electroacupuncture on visceral hypersensitivity of IBS rat models and expressions of PAR4 and TRPV1 in IBS rat models＇ colon and discuss the mechanism of IBS＇s acupuncture treatment. Methods： To set Wistar adult rats as the object of the study and establish models of irritable bowel syndrome rats. The experiment included normal group,model group,EA Dachangshu group and EA Shangjuxu group. The EA Shangjuxu group and EA Dachangshu group were started to be bounded and given EA once in one day,a total of seven times. Model group was bounded but not given EA. The normal group was give nothing intervention. After treatment,observe the stool consistency and detection of fecal water content. Visceral hypersensitivity was used to evaluate the therapeutic effect of electroacupuncture. And then collect colon tissues and measure expression levels of PAR4 and TRPV1 in rats＇ colon tissue by western-blotting method. Results： The model group＇s stool score and fecal water content were significantly higher than those of the normal control group,EA Dachangshu group and EA Shangjuxu group＇s stool score and fecal water content were significantly lower than those of the model group. The difference was statistically significant. Compared with the normal group,pain threshold of the model group was significantly decreased. Compared with the model group,the pain threshold of electroacupuncture treatment group was significantly increased. Compared with the normal group,PAR4 and TRPV1 in the colon of model group were significantly increased. Compared with the model group,PAR4 and TRPV1 in the colon of EA Shangjuxu group and EA Dachangshu group were significantly reduced. Conclusion： Acupuncture can treat constipation-predominant irritable bowel syndrome by adjusting PAR4 and TRPV1 expressions in rats＇ colon tissue.

关 键 词：电针 肠易激综合征 内脏痛敏 PAR4 TRPV1 

分 类 号：R245.97[医药卫生—针灸推拿学] R574.4[医药卫生—中医临床基础]