补肾、健脾、活血法对绝经后骨质疏松症大鼠骨骼、骨骼肌印第安刺猬蛋白(Ihh)含量影响的比较研究  

Effect of Tonifying Kidney,Invigorating Spleen and Promoting Blood Circulation on Protein Expression of Ihh in Bone And Skeletal Muscle Tissues of Rats with Osteoporosis

作　　者：姜奥[1] 金成日[2] 杨芳[3] 

机构地区：[1]沈阳市第五人民医院,辽宁沈阳110023 [2]辽宁中医药大学附属第四医院,辽宁沈阳110100 [3]辽宁中医药大学,辽宁沈阳110847

出　　处：《中华中医药学刊》2017年第9期2392-2394,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省自然科学基金项目(201602499)

摘　　要：目的:观察中医不同治法对骨质疏松症大鼠骨骼、骨骼肌印第安刺猬蛋白(Ihh)含量变化的影响,为临床中医防治骨质疏松症的提供理论依据。方法:将SD雌性大鼠分为正常组、模型组、补肾组、健脾组、活血组。采用酶联免疫法测定小鼠骨骼、骨骼肌Ihh含量浓度变化。结果:(1)与正常组比较,模型组大鼠骨骼的Ihh含量降低明显(P<0.01);与模型组比较,补肾组和健脾组骨骼的Ihh含量明显升高(P<0.01)。(2)与正常组比较,模型组大鼠骨骼肌的Ihh含量显著降低(P<0.01);与模型组比较,补肾组和健脾组骨骼肌的Ihh含量明显升高(P<0.01)。结论:(1)骨质疏松症的形成,可能与骨骼、骨骼肌协调性下降有关。(2)补肾、健脾方法通过提高骨质疏松症大鼠的骨骼、骨骼肌Ihh含量,对骨质疏松症具有一定的防治作用。Objective： To observe the effects of tonifying kidney,invigorating spleen and promoting blood circulation on protein expression of of Ihh in bone and skeletal muscle tissues of the rats with osteopotosis and discuss the prevention and treatment of osteoporosis of TCM. Methods： Rats were randomly divided into normal control group,model control group,tonifying kidney group group,invigorating spleen group and promoting blood circulation group. The protein expression of Ihh was determined by ELISA method. Results：（1）Compared with normal group,the content of Ihh enzyme in bone tissues of model control group decreasd（ P〈0. 01）. Compared with model control group,the content of Ihh enzyme in bone tissues of tonifying kidney group and invigorating spleen group increased.（2）Compared with normal group,the content of Ihh enzyme in skeletal muscle tissues of model control group decreasd（ P〈0. 01）. Compared with model control group,the content of Ihh enzyme in skeletal muscle tissues of tonifying kidney group and invigorating spleen group increased. Conculsion：（1）Osteoprosis may be related to the harmony of bone and skeletal muscle.（2）The method of tonifying kidney and invigorating spleen have certain preventive effects on osteoporosis by regulating the protein expression of Ihh in bone and skeletal muscle tissues.

关 键 词：骨质疏松症 印第安刺猬蛋白 补肾 健脾 

分 类 号：R285.5[医药卫生—中药学]