玉米泛素结合酶基因ZmUBC-76的功能分析  

Functional Analysis of a Ubiquitin-conjugating Enzyme Gene ZmUBC-76 in Maize

作　　者：决登伟[1] 桑雪莲 舒波[1] 刘丽琴[1] 王一承[1] 石胜友[1] 

机构地区：[1]中国热带农业科学院南亚热带作物研究所农业部热带果树生物学重点实验室,广东湛江524091

出　　处：《热带作物学报》2017年第8期1507-1511,共5页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金(No.20163111);国家自然科学基金项目(No.31572087)

摘　　要：通过生物信息学方法,从玉米基因组中得到1个泛素结合酶家族基因序列,将其命名为ZmUBC-76。该基因序列开放阅读框为2 589 bp,编码的蛋白具有862个氨基酸,其分子量为98.91 ku,理论等电点为8.07。预测定位于细胞核。荧光定量PCR分析表明,该基因具有组织表达特异性,在根和幼果中的表达量最高,在穗丝中表达最低。非生物胁迫表达分析结果表明:在盐胁迫和干旱胁迫下,ZmUBC-76的表达呈逐渐下降趋势,在处理24 h时达到最低;在低温胁迫时,ZmUBC-76的表达量未出现显著变化。上述结果表明,ZmUBC-76可能参与了植物对盐和干旱胁迫的响应。In the present study, a ubiquitin-conjugating family gene was identified and designated as ZmUBC-76, in maize genome by using the bioinformatics method. The ORF of ZmUBC-76 is 2 589 bp that encodes 862 amino acid residues with an estimated molecular mass of 98.91 ku and a calculated pI of 8.07. Meanwhile, Plant-mPLoc predicted that the ZmUBC-76 protein was localized in the nucleus. Real-time qPCR assay showed that the highest expression level of ZmUBC-76 occurred in the roots and young seeds, and the lowest of ZmUBC-76 occurred in the silks, which meant ZmUBC-76 appeared to have a tissue-specific expression pattern. The expression levels of ZmUBC-76 under different abiotic stress were also analyzed by real-time qPCR. The results showed that the expression of ZmUBC-76 decreased at the test time by salt and drought stress, and reached the lowest level at 24 h. However, the expression levels of ZmUBC-76 under low temperature stress had no significant change. These results indicated that ZmUBC-76 could be involved in plant response to salt and drought stress.

关 键 词：玉米 泛素结合酶基因 干旱 低温 盐胁迫 

分 类 号：Q78[生物学—分子生物学]