骨化三醇可抑制大鼠心肌间质成纤维细胞增殖  

Study on calcitriol in inhibiting the proliferation of mycordial intersitical fibroblasts in rats

作　　者：邹力勇[1] 胥光热[2] 刘丽[3] 

机构地区：[1]宜宾市第二人民医院药剂科,四川宜宾644000 [2]四川省人民医院消化内科,四川成都610072 [3]宜宾市第二人民医院心血管内科,四川宜宾644000

出　　处：《西部医学》2017年第9期1208-1210,1216,共4页Medical Journal of West China

摘　　要：目的探讨骨化三醇对血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)诱导的大鼠心肌间质成纤维细胞增殖及其机制。方法体外培养大鼠心肌间质成纤维细胞,分别以终浓度为10-6 mol/L AngⅡ和(或)终浓度为10-6 mol/L、10-7 mol/L、10-8 mol/L的骨化三醇进行干扰(骨化三醇组),对照组则在心肌间质成纤维细胞培养液中不加入干扰因素。WST-1法检测心肌间质成纤维细胞数目;流式细胞仪检测细胞周期。结果骨化三醇能抑制基础和AngⅡ刺激状态下的大鼠心肌间质成纤维细胞增殖,经不同浓度的骨化三醇(10-6 mol/L、10-7 mol/L、10-8 mol/L)作用48小时后,细胞G1期细胞较AngⅡ组明显增加,分别为22.%、13%、11%(P均<0.05),并在10-6 mol/L、10-7 mol/L、10-8 mol/L范围内呈浓度依赖性显著降低。结论骨化三醇通过使大鼠心肌间质成纤维细胞停滞在G1期而达到抑制细胞的活化及增殖,具有抗心肌间质成纤维细胞转分化的潜在作用。Objective To observe the effects of calcitriol on cellular proliferation in cardial fibroblasts induced by angiotenin II and explore its potenial roles and underlying mechanisms in preventing myocardial interstitial fibrosis.Methods Myocardial fibroblasts were cultured in vitro. and inferfered with AngII of final concentration in 10^-6mol/L and calcitriol of final concentration in 10^-6mol/L、10^-7mol/L and 10^-8mol/L.The cellular proliferation was detected by WST.1. The cellular cycle was assessed by flow cytometry using annexinV FITC.Results Calcitriol can inhibit the cellular proliferation in the condition of basic simulating by AngⅡ.G1-arrest significantly increased 22%.13% and 11% （P〈0.05）,compared with that of AngII group after 48 hours＇ action of different concentration calcitriol （10^-6mol/L, 10^-7mol/L and 10^-8mol/L）. The concentration dependence of calcitrial significantly decreased within the scope of 10^-6mol/L, 10^-7mol/L and 10^-8mol/L .Cellular proliferation was inhibited by calcitriol through Glarrest（P〈0.05）. Conclusion Calcitriol inhibits cellular proliferation of myocardial interstitial fibroblast by G1-arrest, and can prevent myocardial interstitial fibroblast caused by angiotensinII.

关 键 词：骨化三醇 血管紧张素Ⅱ 心肌间质成纤维细胞 

分 类 号：R542.2[医药卫生—心血管疾病]