Micro-CT观察重组人甲状旁腺素在骨折愈合过程中的作用  

Effect of recombinant human parathyroid hormone on bone fracture healing in the rat observed by micro-CT

作　　者：吴成爱[1] 阎国强[1] 李宁[2] 姜旭[3] 赵丹慧[1] 

机构地区：[1]北京市创伤骨科研究所,北京100035 [2]北京积水潭医院创伤骨科,北京100035 [3]北京积水潭医院矫形骨科,北京100035

出　　处：《中国实验动物学报》2017年第4期350-355,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(编号:81071499;81330043)

摘　　要：目的使用Micro-CT,探讨重组人甲状旁腺激素(rh PTH)在2月龄雄性大鼠骨折愈合过程中的作用。方法应用2月龄雄性SD大鼠建立单侧闭合性股骨骨折内固定模型,随机分为甲状旁腺素治疗组和对照组,术后分别皮下注射重组人甲状旁腺素(1-34)10μg/(kg.d)或等剂量生理盐水安慰剂(对照组),分别于术后第2、7、14、21和42天取双侧股骨标本,分别行X线检查和Micro-CT观察,并进行骨结构的定量分析。结果术后第21天,甲状旁腺素组骨折断端两侧的骨痂之间连续性良好,骨折线已模糊不清。甲状旁腺组较对照组,在术后第42天骨体积(bone Volume,BV)、骨体积分数(BV/TV)、骨密度(bone mineral density,BMD)和骨小梁模式因子(trabecular bone pattern factor,Tb.Pf)明显高于对照组;而骨小梁分离度(trabecular separation,Tb.Sp)和各向异性的程度(degree of anisotropy,DA)低于对照组。结论低剂量重组人甲状旁腺激素可提高大鼠骨折BV、BV/TV、Tb.Pf和BMD而降低Tb.Sp和DA,从而促进骨折的愈合。Objective To investigate the accelerating role of recombinant human parathyroid hormone（ PTH） in bone fracture repair. Methods 2-month old male Sprague-Dawley rats underwent closed unilateral femoral fracture and intramedullary nail fixation. The rats were divided into 2 equal groups randomly： the treatment group receiving subcutaneous injection of rh PTH（ 1-34） 10 μg/（ kg·d） immediately after operation and for 2,7,14,21 and 42 d,respectively,and the control group receiving subcutaneous injection of normal saline in the same volume. X-ray and micro-CT were conducted at 2,7,14,21 and 42 days after surgery. Results The continuity of porosis between fracture sides was better and fracture line has been blurred in the PTH-treated group at 21 days after fracture compared with the control group,the bone volume（ BV）,BV/TV,bone mineral density（ BMD） and trabecular pattern factor（ Tb. Pf） were significantly higher,and trabecular separation（ Tb. Sp） and degree of anisotropy（ DA） were significantly lower in the PTH-treated group at 42 days after fracture. Conclusions Our findings suggest that a low dose recombinant human parathyroid hormone can accelerate the bone fracture healing,probably through improving the BV,BV/TV,Tb. P and BMD,and decreasing the Tb. Sp and DA..

关 键 词：骨折愈合 甲状旁腺激素 骨体积 骨小梁模型因子 骨密度 大鼠 

分 类 号：Q95-33[生物学—动物学]