EGCG和EGCG-3Me对根管牙本质粘接界面稳定性的作用  

Effects of EGCG and EGCG-3Me on the bonding stability to intraradicular dentin

作　　者：余昊翰 张凌 李芳 刘正雅 李银花[2] 陈吉华 YU Hao-han ZHANG Ling LI Fang LIU Zheng-ya LI Yin-hua CHEN Ji-hua(State Key Laboratory of Military Stomatology＆National Clinical Research Center for Oral Diseases＆Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi＇an, Shaanxi 710032, China National Research Center of Engineering Technology for Utilizations of Functional Ingredients from Botanicals, Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China)

机构地区：[1]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学口腔医院修复科,陕西西安710032 [2]国家植物功能成分利用工程技术研究中心,湖南农业大学茶学教育部重点实验室,湖南长沙410128

出　　处：《粘接》2017年第9期17-21,33,共6页Adhesion

基　　金：国家自然科学基金(No.51373198;81470773;81571019);陕西省重点研发计划项目(2017SF-115);长江学者和创新团队发展计划(IRT13051)

摘　　要：将质量浓度为400μg/mL的EGCG及EGCG-3Me添加到全酸蚀粘接剂Single Bond 2(SB2)中,制备改性粘接剂E-SB2及E3-SB2,SB2为对照组。激光共聚焦显微镜和分光光度法检测改性粘接剂抗粪肠球菌的性能;微拉曼光谱仪检测粘接剂双键转化率;制备纤维桩粘接试件,用于即刻和老化后的微推出实验。结果表明,改性粘接剂可以抑制粪肠球菌生物膜形成,且EGCG-3Me作用更显著;改性粘接剂与SB2的双键转化率和即刻微推出粘接强度差异无显著性(P>0.05);老化后改性粘接剂的微推出粘接强度显著高于SB2(P<0.05)。Epigallocatechin-3-gallate （EGCG） and epigallocatechin-3-O-（3-O-methyl）-gallate （ EGCG-3Me ） was incorporated into the total-etch adhesive of Single Bond 2 separately to obtain two modified adhesives E-SB2 and E3- SB2 with the concentration of 400 μg/mL of EGCG and EGCG-3Me respectively, The confocal laser scanning microscopy （CLSM） and the ultraviolet spectrophotometry were used to evaluate the anti-bacterial effect of modified adhesives. The micro-Raman spectrum was used to test the degree of double bond conversion （DC） of adhesives. The push-out bond strength test was conducted to test the immediate bonding strength and the bonding strength after thermocycling. The results demonstrated that E-SB2 and E3-SB2 both showed inhibiting effect to the growth and activity of E.faecalis, while E3-SB2 performed stronger inhibiting effect. The DC and immediate push-out bonding strength of SB2 were not decreased with the incorporation of EGCG or EGCG-3Me （P〉0.05）. E-SB2 and E3-SB2 showed significantly higher push-out bonding strengths than that of SB2 （P 〈 0.05）.

关 键 词：EGCG EGCG-3Me 粘接剂 粘接稳定性 

分 类 号：TQ437[化学工程]