激光共聚焦显微镜在牙本质粘接界面观察中的应用  

Observation of the dentin-adhesive interface using confocal laser scanning microscopy

作　　者：周唯 陈吉华 ZHOU Wei CHEN Ji-hua(State Key Laboratory of Military Stomatology＆National Clinical Research Center for Oral Diseases＆Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi＇an, Shaanxi 710032, China)

机构地区：[1]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学口腔医院修复科,陕西西安710032

出　　处：《粘接》2017年第9期22-26,共5页Adhesion

基　　金：国家自然科学基金(81470773);教育部创新团队及长江学者奖励计划(No.IRT13051)

摘　　要：先制备牙本质粘接试件,将切片打磨后使用激光共聚焦显微镜对牙本质粘接界面的自发荧光特征进行探测。不同粘接剂形成的粘接界面都表现出了特异性的自发荧光现象,不同结构区分明显。这种激光共聚焦显微镜探测自发荧光有可能成为一种新的牙本质粘接界面观察分析方法。The dentin bonding specimens were prepared according to instruction of manufactures. Then bonding specimens were cut into the slices and polished. Then all the specimens were observed by the confocal laser scanning microscopy under the same condition. The results showed that the dentin-adhesive interfaces formed by different bonding agents exhibited special auto-fluorescence. Identification of different structure in the bonding interface was distinctive. So the observation of auto-fluorescence by confocal laser scanning microscopy could be a novel method for the analysis to the dentin-adhesive interface.

关 键 词：牙科粘接剂 牙本质粘接界面 自发荧光 激光共聚焦显微镜 

分 类 号：TQ430[化学工程]