基于Ru(bpy)_3^(2+)/AuNPs/SWCNTs/腺苷适配体电化学发光生物传感器用于腺苷的检测  

Determination of Adenosine Based on Ru(bpy)_3^(2+)/AuNPs/SWCNTs/Adenosine Aptamer Electrochemiluminescence Sensor

作　　者：樊雪梅[1] 王书民[1] 李哲建[1] 贾雪艳[2] 郑行望[3] 

机构地区：[1]商洛学院化学工程与现代材料学院/陕西省尾矿资源综合利用重点实验室,商洛726000 [2]西北师范大学化学化工学院,兰州730070 [3]陕西师范大学化学化工学院,西安710062

出　　处：《分析化学》2017年第9期1353-1359,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.30970696);陕西省科技统筹创新工程计划项目(No.2012KTDZ02-02);陕西省教育厅项目(No.16JS028);商洛市科技局项目(Nos.SK-2014-1;SK2015-37);商洛学院项目(No.15sky024)资助~~

摘　　要：利用AuNPs/Nafion复合膜技术固定Ru(bpy)_3^(2+),采用羧基化碳纳米管固定氨基化腺苷适配体,制备腺甘电化学发光生物传感器。采用循环伏安法和电化学发光法对传感器进行表征。结果表明,此传感器具有良好的稳定性和重现性。腺苷与传感器作用后,腺苷与其适配体形成G四面体结构,Ru(bpy)_3^(2+)的电化学发光强度降低。在最佳实验条件下,电化学发光强度降低量与腺苷浓度的负对数在1.0×10 ^(-11)~1.0×10^(-7)mol/L范围内呈良好的线性关系,线性方程为ΔI_(ECL)=-890lg C-5050,检出限(S/N=3)为5.0×10^-(12)mol/L。对1.0×10^(-10)mol/L腺苷平行测定11次,相对标准偏差为2.7%。用于尿液中腺苷的测定,加标回收率在97.1%~110.0%之间。Based on the Au NPs/Nafion composite membrane technology and immobilization of amino adenosine aptamer using carboxyl carbon nanotubes on the surface of a glassy carbon electrode, a electrochemiluminescence sensor was preparated. The sensor was characterized by cyclic voltammetry and electrochemical luminescence. The result showed that the sensor had a good stability and reproducibility.Adenosine and adenosine aptamer could form G-tetrahedral structure,leading a decrease of ECL intensity.Under the optimum experimental conditions,the relative ECL intensity showed a good linear relationship to the negative logarithm of adenosine concentration in the range of 1. 0 ×10-11-1. 0 ×10-7mol/L,the linear equations was ΔIECL= -890lg C-5050 with a detection limit of 5. 0 × 10-12mol/L. The RSD was 2. 7% in 11 times measurement of adenosine（ 1. 0 ×10-10mol/L）. The recovery was 97. 1%-110. 0% in the determination of real adenosine sample.

关 键 词：电化学发光 腺苷适配体 腺苷 

分 类 号：O657[理学—分析化学] TP212.3[理学—化学]