基于抗原决定基的胰岛素分子印迹电化学传感器  

An Insulin Molecularly Imprinted Electrochemical Sensor Based on Epitope Imprinting

作　　者：赵成军[1] 马雄辉[1] 李建平[1] 

机构地区：[1]广西电磁化学功能物质重点实验室桂林理工大学化学与生物工程学院,桂林541004

出　　处：《分析化学》2017年第9期1360-1366,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21375031;21765006)项目;广西自然科学基金创新研究团队项目(No.2015GXNSFFA139005);广西高等学校高水平创新团队及卓越学者计划项目(桂教人[2014]49号)资助~~

摘　　要：采用抗原决定基法制备了胰岛素电化学分子印迹传感器。以胰岛素C端多肽作为模板分子,定向自组装在Au电极上,以邻苯二胺为功能单体,电化学聚合制备分子印迹聚合膜。以Na OH为洗脱液,洗脱模板分子,形成的与胰岛素C端多肽三维结构相匹配的分子印迹孔穴能特异性识别胰岛素。重吸附胰岛素分子后,以K_3[Fe(CN)_6]/K_4[Fe(CN)_6]为探针,通过测量探针在电极表面产生的电流大小实现胰岛素的间接测定。在1.0×10^(-14)~5.0×10^(-13)mol/L浓度范围内,传感器的电流响应值与胰岛素浓度呈良好的线性关系,检出限为7.24×10^(-15)mol/L(3σ)。此传感器具有较好的选择性和稳定性,并成功用于血清样品中胰岛素的测定。A novel molecularly imprinted electrochemical sensor for direct detection of insulin was prepared based on epitope imprinting. C-Terminal polypeptide in insulin as template molecule was firstly self-assembled on the Au electrode. Then the molecularly imprinted polymer（ MIP） was fabricated by electropolymerization with o-phenylenediamine（ o-PD） as functional monomer on this Au electrode. After elution of template molecules by Na OH solution,the imprinting cavities were formed with the three-dimensional structure matched with the polypeptide in insulin molecules. The imprinting cavities could specifically recognize and rebind with insulin molecules. With K3[Fe（ CN）6]/K4[Fe（ CN）6] as a probe,the insulin was indirectly detected.There was a linear relationship between the response current and the insulin concentrations in the range of1. 0 × 10-14-5. 0 × 10-13mol/L,and the detection limit was 7. 24 × 10-15mol/L. The developed sensor exhibited good selectivity and stability,and could be applied to the determination of serum samples.

关 键 词：胰岛素 分子印迹 电化学传感器 抗原决定基法 

分 类 号：O657.1[理学—分析化学] TP212.3[理学—化学]