水体总毒性在线检测仪器的研制  

An Online Equipment for Monitoring of Water Total Toxicity

作　　者：翟俊峰[1] 余登斌[1] 董绍俊[1] 

机构地区：[1]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022

出　　处：《分析化学》2017年第9期1415-1419,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21675151;21405148);科技部项目(Nos.2016YFA0203200;2013YQ17058501);吉林省科技厅项目(No.20150520010JH)资助~~

摘　　要：研制了基于电化学媒介体法的水体总毒性在线检测仪器,通过对比水样和标样中微生物的呼吸活性差异,实现对水体总毒性的在线监测。毒性检测的受试体微生物为原位培养微生物。电化学检测采用三电极系统,其中铂微阵列电极、Ag/Ag Cl(3 mol/L KCl)电极和金丝电极分别作为工作电极、参比电极和对电极。对浓度为5.0、10.0和20.0 mg/L的模型毒物3,5-二氯苯酚(DCP)进行检测,获得的微生物呼吸活性抑制率分别为24.4%、48.9%和59.2%。在连续在线监测过程中,仪器能够对所有随机加入毒性物质(5.0 mg/L DCP)的水样输出毒性警报(以10%的呼吸抑制率为毒性报警临界值),表明此仪器可以用于水体总毒性在线检测和预警。The monitoring of total toxicity of water is of great importance to guarantee the safety of drinking water and estimate the level of water pollution from multiple pollutants. Here, we reported an online equipment for monitoring toxicity of water,where in-site cultured microorganisms were used as the test organism and respiratory inhibition of microorganisms was regarded as the toxicity response. A three-electrode electrochemical detector was integrated in the equipment,where an array electrode composed of microsized Pt disks,a Ag/Ag Cl（ 3 mol/L KCl） electrode and a gold wire were used as the working,reference and counter electrodes,respectively. The average respiratory inhibition ratios obtained with this equipment for 5. 0,10. 0,20. 0 mg/L 3,5-dichlorophenol（ DCP） were 24. 4%,48. 9% and 59. 2%,respectively. The equipment was further used for continuous online monitoring of the total toxicity of underground water,and successfully showed the toxicity alarm when challenged randomly by toxicity samples containing 5. 0 mg/L DCP. The present results suggested the good promise of the equipment in online monitoring of water total toxicity.

关 键 词：水体急性毒性 在线检测 微生物 呼吸抑制 

分 类 号：TH83[机械工程—仪器科学与技术] X853[机械工程—精密仪器及机械]