红毛七的抗炎、镇痛作用研究  

Study on anti-inflammatory and analgesic effects of Caulopyhllum robustum

作　　者：李晓春 王莉芳 徐长根 樊宝娟 LI Xiaochun;WANG Lifang;XU Changgen;FAN Baojuan(Shaanxi Institute for Food and Drug Control,Shaanxi Province,Xi'an 710065,China)

机构地区：[1]陕西省食品药品监督检验研究院,陕西西安710065

出　　处：《中国医药导报》2017年第25期26-29,共4页China Medical Herald

基　　金：陕西省食品药品检验所青苗基金(SQM-201202);陕西省中医管理局中医药科研课题(ZY-24)

摘　　要：目的考察红毛七的抗炎、镇痛作用。方法将小鼠按体重随机分为模型对照组(CMC-Na),阳性对照组,红毛七水提取物低、中、高剂量组(3、9、27 g/kg,以生药计),每组各10只,灌胃给药,每天1次,连续7 d。采用二甲苯致小鼠耳肿胀法测定小鼠耳肿胀度;采用纸片致肉芽增生模型,测定小鼠肉芽肿抑制率,以考察红毛七水提取物的抗炎作用;采用乙酸扭体法测定小鼠扭体反应潜伏期及15 min内扭体次数;采用热板法测定小鼠给药前及给药30、60、90、120 min后痛阈值,以考察红毛七水提取物的镇痛作用。结果与模型对照组比较,红毛七水提取物中、高剂量组可减轻小鼠耳肿胀度(P<0.05或P<0.01),高剂量组可以减轻肉芽组织干重(P<0.05),红毛七水提取物中、高剂量组可延长小鼠扭体反应潜伏期并减少扭体次数(P<0.05或P<0.01),提高给药30、60 min后的痛阈值(P<0.05或P<0.01),且高剂量组可以提高给药90、120 min后的痛阈值(P<0.05或P<0.01)。结论红毛七水提取物有一定的抗炎、镇痛作用。Objective To investigate the anti-inflammatory and analgesic effects of Caulopyhllum robustum water extract. Methods The mice were randomly by weight divided into model group （CMC-Na）, positive control group, Caulopyhllum robustum water extract low-dose, medium-dose and high-dose groups （3, 9, 27 g/kg, calculated by crude drug）, each group had 10 mice. They were given relevant medicine intragastrically, once a day, for consecutive 7 d. The xylene-in- duced ear edema method was used to determine the degree of ear edema. Paper-induced granuloma model was used to determine the inhibition ratio of granulation tissue hyperplasia, the anti-inflammatory activity of Caulopyhllum robustum water extract was investigated. The pain incubation period and the number of writhing within 15 minutes were determined by acetic acid writhing method. Pain thresholds of mice were determined by hot-plate test before and after medication 30, 60, 90, 120 minutes, the analgesic effect of Caulopyhllum robustum water extract was investigated. Resuits Compared with model group, Caulopyhllum robustum water extract medium-dose and high-dose groups could re strain the degree of ear edema of mice （P 〈 0.05 or P 〈 O.01）; the high-dose group could decrease the dry weight of granulation tissue （P 〈 0.05）. Caulopyhllum robustum water extract medium-dose and high-dose groups could prolong the pain incubation period, decrease the number of writhing in mice within 15 minutes （P 〈 0.05 or P 〈 0.01）, and extended pain thresholds of mice 30, 60 minutes after medication （P 〈 0.05 or P 〈 0.01）, the high-dose group could also extend pain thresholds of mice 90, 120 minutes after medication （P 〈 0.05 or P 〈 0.01）. Conclusion CaulopyhUum robustum water extract possesses anti-inflammatory and analgesic effects.

关 键 词：红毛七 水提取物 抗炎 镇痛 动物药效学 

分 类 号：R285.5[医药卫生—中药学]