Hydrophobic interaction membrane chromatography for bioseparation and responsive polymer ligands involved  

作　　者：Jingling CHEN Rong PENG Xiaonong CHEN 

机构地区：[1]Beijing Laboratory of Biomedical Materials, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China

出　　处：《Frontiers of Materials Science》2017年第3期197-214,共18页材料学前沿（英文版）

基　　金：Acknowledgement The authors would like to thank the National Natural Science Foundation of China （Grant No. 20874004） for financial support.

摘　　要：Hydrophobic interaction chromatography （HIC） is a rapid growing bioseparation technique, which separates biomolecules, such as therapeutic proteins and antibodys, based on the reversible hydrophobic interaction between immobilized hydrophobic ligands on chromatographic resin spheres and non-polar regions of solute molecule. In this review, the fundamental concepts of HIC and the factors that may affect purification efficiency of HIC is summarized, followed by the comparison of HIC with affinity chromatography and ion-exchange chromatography. Hydrophobic interaction membrane chromatography （HIMC） combines the advantages of HIC and membrane process and has showed great potential in bioseparation. For better understanding of HIMC, this review presents an overview of two main concerns about HIMC, i.e. membrane materials and hydrophobic ligands. Specifically, cellulose fiber-based membrane substrate and environment-responsive ligands are emphasized.Hydrophobic interaction chromatography （HIC） is a rapid growing bioseparation technique, which separates biomolecules, such as therapeutic proteins and antibodys, based on the reversible hydrophobic interaction between immobilized hydrophobic ligands on chromatographic resin spheres and non-polar regions of solute molecule. In this review, the fundamental concepts of HIC and the factors that may affect purification efficiency of HIC is summarized, followed by the comparison of HIC with affinity chromatography and ion-exchange chromatography. Hydrophobic interaction membrane chromatography （HIMC） combines the advantages of HIC and membrane process and has showed great potential in bioseparation. For better understanding of HIMC, this review presents an overview of two main concerns about HIMC, i.e. membrane materials and hydrophobic ligands. Specifically, cellulose fiber-based membrane substrate and environment-responsive ligands are emphasized.

关 键 词：hydrophobic interaction membrane chromatography BIOSEPARATION MEMBRANE environmental response ligand 

分 类 号：Q503[生物学—生物化学] S432.4[农业科学—植物病理学]