建立检测制式长枪上DNA多态性的新方法  

A new methodology of DNA polymorphism detection from standard long gun

作　　者：杨秀乔 苗琳 谢洪良 黄磊 苏世达 潘顺勇 曾发明[6] 翟滇[6] 程宝文[1,6] 

机构地区：[1]昆明医科大学法医学院,云南昆明650500 [2]玉溪市公安局,云南玉溪653100 [3]曲靖市公安局,云南曲靖655000 [4]昆明市公安局,云南昆明650034 [5]迪庆州公安局,云南迪庆674400 [6]云南省公安厅刑事科学技术研究所,云南昆明650500

出　　处：《中国法医学杂志》2017年第4期385-387,392,共4页Chinese Journal of Forensic Medicine

基　　金：云南省科技惠民计划(2013CA013)

摘　　要：目的建立快速、准确检测制式长枪上基因分型的新方法。方法联合使用直扩法、硅膜法对48支制式长枪上5个不同部位共240份检材进行DNA检测。结果联合使用直扩法及硅膜法,在48支制式长枪中检测出DNA完整分型42支,检出率达87.50%。结论联合使用直扩法、硅膜法,可有效快速、准确地检测出制式长枪上DNA多态性。Objective A new methodology was established to efficiently obtain the genotype of DNA remained on standard long gun. Methods Direct PCR and silicon membrane method were combined to detect DNA polymorphism of a total of 240 samples at 5 different positions from 48 standard long guns. Results Combining direct PCR and silicon membrane method, we obtained full DNA profiles in 42 out of 48 standard long guns, with a detection rate up to 87.50%. Conclusion The results demonstrate that the combination of direct PCR and silicon membrane method provide a quick and accurate way to detect DNA polymorphism on the standard long gun.

关 键 词：法医物证学 直扩法 硅膜法 制式长枪 基因分型 

分 类 号：D919.4[医药卫生—法医学]