HPLC测定显齿蛇葡萄茎中3种黄酮类成分的含量  

Determination of Contents of Dihydromyricetin, Rutin and Myricetin in Ampelopsis Grossedentata Stems by HPLC

作　　者：常敬芳 覃洁萍[1] 刁宇[1] 黄志飘 刘鹏飞[1] 

机构地区：[1]广西中医药大学药学院,南宁530001

出　　处：《中国现代应用药学》2017年第8期1154-1157,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：广西科学研究与技术开发计划项目(桂科重1598005-10);广西壮族自治区卫生厅中医药科技专项(GZBZ14-08);广西壮药材质量评价与标准研究专项(GXZC2014-G3-1577-YLZB-B-3);广西高校科技创新能力提升项目专项(J17002)

摘　　要：目的建立显齿蛇葡萄茎中黄酮类成分的含量测定方法,分析广西不同地区显齿蛇葡萄茎中二氢杨梅素、芦丁及杨梅素的含量。方法采用色谱柱为Poroshell EC-C_(18)(4.6 mm×250 mm,4.0μm),以乙腈-0.1%磷酸为流动相,梯度洗脱,流速1 m L·min^(-1),柱温25℃,检测波长0~23 min,330 nm;23~50 min,375 nm。结果显齿蛇葡萄茎中二氢杨梅素、芦丁及杨梅素进样量分别在0.800 5~9.606μg(r=0.999 9)、0.201 6~2.419μg(r=0.999 8)和0.100~1.202μg(r=0.999 7)内呈良好的线性关系;平均加样回收率分别为98.1%,101%和99.9%,RSD<3%,方法的稳定性、重复性和精密度均良好(RSD<3%)。桂林灵川的显齿蛇葡萄茎中二氢杨梅素、芦丁和杨梅素的含量均较高,武鸣双江和金秀互助村的显齿蛇葡萄茎中二氢杨梅素和杨梅素含量均较低,而武鸣双江的芦丁含量较高,柳州泗顶镇的芦丁含量较低。结论不同地区显齿蛇葡萄茎中二氢杨梅素、芦丁和杨梅素的含量相差较大。该方法结果准确、重复性好,可使目标成分与试样中其他共存组分达到较好地分离。OBJECTIVE To establish a method for the determination of contents of flavonoids and analyze the contents of dihydromyricetin, rutin and myricetin in Ampelopsis grossedentata stems from different areas of Guangxi. METHODS HPLC method was adopted. The separation was performed on Agilent Poroshell EC-C_（18）（4.6 mm×250 mm, 4.0 μm） column and the mobile phase consisted of acetonitrile and 0.1% H_3PO_4 water solution in gradient elution at flow rate of 1 m L·min^（-1）, the column temperature was 25 ℃, the detection wavelengths were set at 330 nm for dihydromyricetin and 375 nm for myricetin. RESULTS The linear ranges were 0.800 5-9.606 μg（r=0.999 9） for dihydromyricetin, 0.201 6-2.419 2 μg（r=0.999 8） for rutin and 0.100-1.202 μg（r=0.999 7） for myricetin, and the average recovery were 98.1%, 101% and 99.9%, respectively, RSDs〈3%. The determined results showed that the contents of dihydromyricetin, rutin and myricetin in Ampelopsis grossedentata stems from Lingchuan of Guilin were higher, and the contents in dihydromyricetin and myricetin in Ampelopsis grossedentata stems from Shuangjiang of Wuming and Huzhu Cun of Jinxiu were lower; the contents of rutin in Ampelopsis grossedentata stems from Shuangjiang of Wuming were higher, and the contents of rutin from Siding Zhen of Liuzhou were lower. CONCLUSION The contents of dihydromyricetin, rutin and myricetin in Ampelopsis grossedentata stems from different areas were quite different. This method can be better to separate from coexisting component, with good separation, and the results showed the method had high accuracy and good reproducibility.

关 键 词：显齿蛇葡萄茎 二氢杨梅素 芦丁 杨梅素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R917.101[医药卫生—中医学]