血液灌流对染毒犬血液中百草枯的清除作用  

Experimental study scavenging effect of paraquat by hemoperfusion

作　　者：裴禹皓 何跃忠[2] 张锡刚[1] 孙百胜[1] 

机构地区：[1]军事医学科学院附属医院急诊科,北京100071 [2]军事医学科学院科技部

出　　处：《中华劳动卫生职业病杂志》2017年第7期523-525,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家科技支撑计划（2012BA138804）

摘　　要：目的探讨百草枯中毒后早期行单次及二次血液灌流（hemoperfusion HP）对染毒犬血液中毒物的清除效果及毒物代谢改变，以探索更优HP方案。方法将18只比格犬随机分为3组：单次HP治疗组、二次HP治疗组及对照组。采集各组比格犬百草枯染毒后48h内不同时间点的外周静脉血，测量、分析并比较血浆中的毒物浓度。结果染毒后6h，单次HP组及二次HP组结束首次HP治疗，PQ浓度明显低于对照组，差异有统计学意义（P〈0．05）；染毒后10h，三组PQ浓度的差异无统计学意义（P〉0．05）；染毒后12h，二次HP组结束第二次HP治疗，其PQ浓度明显低于单次HP组及对照组，差异有统计学意义（P〈0．05）；染毒后24h及48h，各组PQ浓度差异无统计学意义（P〉0．05）。各组在毒代动力学参数比较，差异无统计学意义（P〉0．05o结论中毒后12h内的单次及二次HP治疗在灌流后短时间内可有效降低血液中的百草枯浓度，但在HP后短时间内恢复至同一水平；提示中毒初期单次或二次HP治疗不能明显改变百草枯的毒物代谢。Objective To determine the scavenging effect and the change of metabolism of paraquat （PQ） using hemoperfusion （HP） once and twice within 12 hours after intoxication and explore the better scheme of HP. Methods 18 beagles were randomly divided into 3 groups. Single HP group , Double HP group and Control group. Peripheral veins blood was collected at different times within 48 hours after exposure in each group. Toxin concentration was measured, analyzed and compared among 3 groups. Results 6 hours after exposure, Single HP group and Double HP group has finished the first HP treatment, and the concentration of PQ was lower than that of the control group, the difference was statistically significant （P〈0.05）. 10 hours after exposure, there was no statistical difference of toxin concentration among 3 groups （P〉0.05）. 12 hours after exposure, Double HP group has finished the second HP treatment, the concentration of PQ was significantly lower than that of Single HP group and Control group （P〈0.05）. 24 hours and 48 hours after exposure, there was no statistical difference of toxin concentration among 3 groups （P〉0.05）. Statistical difference were not observed in toxicokinetical parameters among 3 groups （P〉0.05）. Conclusion HP treatment once and twice within 12 hours after intoxication could effectively reduce the toxin concentration in the peripheral veins blood after HP for about 4 hours, then the toxin concentration would return to the same level as Control group quickly. It was suggested that at the beginning of poisoning, HP treatment once or twice could not significantly change the metabolism of paraquat.

关 键 词：血液灌流 百草枯 比格犬 

分 类 号：R595.4[医药卫生—内科学]