飞机噪声刺激引起小鼠初级听皮层神经元凋亡  

Aircraft noise stimulation causes the apoptosis of mouse primary auditory neurons in cortex

作　　者：苏玉婷[1] 孟星星 程耀萍 张海军[1] 谢小萍[1] 常耀明[1] 暴军香[1] Su Yuting Meng Xingxing Cheng Yaoping Zhang Haijun Xie Xiaoping Chang Yaoming Bao Junxiang(Department of Aerospace Hygiene, The Fourth Military Medical University, Xi＇an 710032, China)

机构地区：[1]第四军医大学航空航天医学系卫生学教研室,西安710032

出　　处：《中国组织化学与细胞化学杂志》2016年第6期512-516,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助(31071045)

摘　　要：目的噪声对人体最直接的危害是听力损伤,以往研究多关注外周听觉器官改变,其对听皮层神经元的影响并不清楚,本研究探讨飞机噪声刺激5天后,小鼠初级听皮层神经元的形态改变和凋亡相关变化。方法采用苏木素-伊红(hematoxylin and eosin,HE)染色检测小鼠神经元形态变化,采用免疫组织化学方法和Western blot检测B淋巴细胞瘤-白血病2蛋白(Bcl-2)、Bcl-2相关X蛋白(Bax)、天冬氨酸蛋白水解酶-3(caspase-3)蛋白表达。结果与对照组相比,噪声刺激可导致小鼠初级听皮层神经元体积变小、分布不均、颜色深染、胞浆不清、核固缩。噪声组小鼠听皮层神经元凋亡比例显著增加,Bax蛋白表达显著增加,而Bcl-2表达无明显改变,Bax/Bcl-2显著增加,同时伴有caspase-3水平增加。结论飞机噪声刺激可导致小鼠听皮层神经元形态异常,出现明显凋亡改变,其机制与Bax/Bcl-2信号通路及caspase-3活性增加有关。Objective The most direct damage caused by noise to human body is hearing loss. Previous researches have paid most of their attention to the changes in peripheral auditory organs, while the effect on auditory cortical neurons is not clear. The present work was designed to investigate the change in the morphology and apoptosis level of mouse primary auditory neurons in cerebral cortex after 5 days＇exposure to aircraft noise. Methods The morphological change of the neurons was observed by Hematoxylin and Eosin （HE） staining. Western blot and immunohistochemistry were performed to detect the expression of B-cell lymphoma-2 （Bcl-2）, Bcl-2 associated X protein （Bax） and Caspase-3. Results Compared to the control group, in the mice exposed to noise, the primary auditory cortical neurons became smaller, unevenly distributed and deeply stained with HE. The cytoplasm was blur and karyopycnosis was observed which worsened with the increase of exposure time. The apoptotic ratio was noticeably higher. The expression of Bax protein increased significantly while that of Bcl-2 showed no obvious change, the ratio of Bax to Bcl-2 thus being much higher. The expression of Caspase-3 protein also increased. Conclusion Stimulation by aircraft noise induces the morphological change and apoptosis of mouse primary auditory cortical neurons. The mechanism might involve the activation of Bax/Bcl-2 pathway and Caspase-3.

关 键 词：噪声 听皮层 神经元 凋亡 

分 类 号：Q189[生物学—神经生物学]